十二指肠溃疡和胃活检组织中毒力相关基因的测定。
Determination of virulence-associated genes in duodenal ulcer and gastric biopsies.
作者信息
Saeidi Yasaman, Pournajaf Abazar, Gholami Mehrdad, Hasannejad-Bibalan Meysam, Yaghoubi Sajad, Khodabandeh Mahmoud, Emadi Behzad, Ferdosi-Shahandashti Elaheh, Rajabnia Ramazan
机构信息
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Med J Islam Repub Iran. 2017 Dec 17;31:95. eCollection 2017.
or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for , and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine and genes in strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran. Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of from the biopsy culture, genomic DNA was extracted and subsequently used to identify and virulence genes using specific primers. The isolation rate of strains was 65.7% (169/257). The frequency of and was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively. In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p<0.05).
幽门螺杆菌（Hp）与消化性溃疡疾病、慢性胃炎、溃疡和胃癌密切相关。已确定了与致病性相关的基因，其中一些似乎与感染的更严重临床后果有关。本研究旨在确定从伊朗德黑兰沙里亚蒂医院转诊的胃十二指肠患者分离的幽门螺杆菌菌株中的[具体基因名称1]和[具体基因名称2]基因。2015年1月至11月期间，在内镜检查时从转诊至伊朗德黑兰沙里亚蒂医院的患者中采集胃活检标本。从活检培养物中分离出幽门螺杆菌后，提取基因组DNA，随后使用特异性引物鉴定[具体基因名称1]和毒力基因。幽门螺杆菌菌株的分离率为65.7%（169/257）。[具体基因名称1]和[具体基因名称2]的频率分别为143（84.6%）、169（100%）、131（77.5%）、97（57.3%）、89（52.6%）和72（42.6%）。在本研究中，观察到从消化性溃疡（PUD）和胃癌（GC）患者分离的研究基因与菌株之间存在显著差异（p<0.05）。
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