[高效液相色谱法测定芦荟中芦荟苷的含量]
[Determination of aloin in aloes by HPLC].
作者信息
Cao Hong, Liu Yun
机构信息
Institute for Drug and Instrument Control of PLA, Beijing 100071, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2003 Apr;28(4):349-51.
OBJECTIVE
To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes.
METHOD
The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm.
RESULT
The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6).
CONCLUSION
The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.
目的
描述一种简单快速的等度反相高效液相色谱法，用于芦荟中芦荟苷的基线分离、鉴定和测定。
方法
分析柱为填充有5微米固定相的ZORBAX SB-C18（4.6毫米×250毫米）。流动相由乙腈-水（25:75）组成；流速为1毫升·分钟-1。进样量为10微升。二极管阵列检测器设定在355纳米。
结果
校准曲线在0.17 - 5.9微克范围内呈线性（r = 0.9999）。该方法的平均回收率为98.6%，相对标准偏差为1.32%（n = 6）。
结论
结果表明该方法可靠且准确。该方法应用于11种不同产地的好望角芦荟和东非芦荟。
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