[高效液相色谱法测定复方龟鹿颗粒中甜菜碱的含量]
[Determnination of betaine in Fufang Guilu granule by HPLC].
作者信息
Xu Ming-Wang, Yuan Xiu-Zhi, Liu Yan-Weng, Shen Xu-Ju
机构信息
Affiliated Liyuan Hospital, Mongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(6):462-4.
OBJECTIVE
To describe a HPLC method for assessing betaine in Fufang Guilu granule.
METHOD
The content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(35:65) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm.
RESULT
The calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5).
CONCLUSION
The results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule.
目的
描述一种用于测定复方龟鹿颗粒中甜菜碱的高效液相色谱法。
方法
采用高效液相色谱法测定复方龟鹿颗粒中甜菜碱苯甲酰甲基溴的含量。分析柱为填充5μm固定相的岛津CLC-ODS柱（6.0mm×150mm）；流动相为乙腈-水（35:65），含0.1mol·L⁻¹高氯酸钠；流速为1mL·min⁻¹；检测波长设定为254nm。
结果
校准曲线在0.09 - 0.585μg范围内呈线性（r = 0.9997）。该方法的平均回收率为98.4%，相对标准偏差为2.5%（n = 5）。
结论
结果表明该方法可靠、准确，可用于复方龟鹿颗粒的质量控制。
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