萨斯喀彻温分枝杆菌新种，一种从人类临床分离株中分离出的新型缓慢生长的暗产色菌，与间介分枝杆菌相关，且对鸟分枝杆菌复合群Accuprobe检测呈阳性。
Mycobacterium saskatchewanense sp. nov., a novel slowly growing scotochromogenic species from human clinical isolates related to Mycobacterium interjectum and Accuprobe-positive for Mycobacterium avium complex.
作者信息
Turenne C Y, Thibert L, Williams K, Burdz T V, Cook V J, Wolfe J N, Cockcroft D W, Kabani A
机构信息
National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada R3E 3R2.
Laboratoire de santé publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.
出版信息
Int J Syst Evol Microbiol. 2004 May;54(Pt 3):659-667. doi: 10.1099/ijs.0.02739-0.
A pigmented, slowly growing Mycobacterium avium complex AccuProbe-positive organism was isolated from the sputum and pleural fluid of a 72-year-old female with bronchiectasis. The unusual morphology of the organism prompted further identification by 16S rRNA gene sequencing, revealing a perfect identity with previously uncharacterized strain Mycobacterium sp. MCRO 8 (GenBank accession no. X93034), with the closest established species by 16S rDNA analysis being Mycobacterium interjectum. HPLC of the organism corresponded to previously obtained patterns identified as M. interjectum-like and, upon sequence evaluation of a selection of strains with a similar profile, more were subsequently identified as MCRO 8. A total of 16 strains isolated from human respiratory samples were evaluated in the characterization of this novel species, for which the name Mycobacterium saskatchewanense sp. nov. is proposed. The type strain is strain 00-250(T) (=ATCC BAA-544(T)=DSM 44616(T)=CIP 108114(T)).
从一名患有支气管扩张症的72岁女性的痰液和胸腔积液中分离出一株色素沉着、生长缓慢的鸟分枝杆菌复合群AccuProbe阳性菌。该菌不寻常的形态促使通过16S rRNA基因测序进行进一步鉴定，结果显示与之前未鉴定的菌株Mycobacterium sp. MCRO 8（GenBank登录号：X93034）完全一致，通过16S rDNA分析确定与之亲缘关系最近的已确定物种是间插分枝杆菌。该菌的高效液相色谱分析结果与之前鉴定为间插分枝杆菌样的图谱相符，在对一系列具有相似图谱的菌株进行序列评估后，更多菌株被鉴定为MCRO 8。共对从人类呼吸道样本中分离出的16株菌株进行了评估，以表征这个新物种，为此提议将其命名为萨斯喀彻温分枝杆菌（Mycobacterium saskatchewanense sp. nov.）。模式菌株为00 - 250(T)（=ATCC BAA - 544(T)=DSM 44616(T)=CIP 108114(T)）。
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