Reddy Gundlapalli S N, Matsumoto Genki I, Schumann Peter, Stackebrandt Erko, Shivaji Sisinthy
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
Department of Environmental and Information Science, Otsuma Women's University, Tamashi, Tokyo 206, Japan.
Int J Syst Evol Microbiol. 2004 May;54(Pt 3):713-719. doi: 10.1099/ijs.0.02827-0.
Thirty-one bacteria that belonged to the genus Pseudomonas were isolated from cyanobacterial mat samples that were collected from various water bodies in Antarctica. All 31 isolates were psychrophilic; they could be divided into three groups, based on their protein profiles. Representative strains of each of the three groups, namely CMS 35(T), CMS 38(T) and CMS 64(T), were studied in detail. Based on 16S rRNA gene sequence analysis, it was established that the strains were related closely to the Pseudomonas fluorescens group. Phenotypic and chemotaxonomic characteristics further confirmed their affiliation to this group. The three strains could also be differentiated from each other and the closely related species Pseudomonas orientalis, Pseudomonas brenneri and Pseudomonas migulae, based on phenotypic and chemotaxonomic characteristics and the level of DNA-DNA hybridization. Therefore, it is proposed that strains CMS 35(T) (=MTCC 4992(T)=DSM 15318(T)), CMS 38(T) (=MTCC 4993(T)=DSM 15319(T)) and CMS 64(T) (=MTCC 4994(T)=DSM 15321(T)) should be assigned to novel species of the genus Pseudomonas as Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov., respectively.
从南极洲不同水体采集的蓝藻席样品中分离出31株属于假单胞菌属的细菌。所有31株分离菌均为嗜冷菌;根据其蛋白质谱,它们可分为三组。对这三组中的每一组的代表菌株,即CMS 35(T)、CMS 38(T)和CMS 64(T),进行了详细研究。基于16S rRNA基因序列分析,确定这些菌株与荧光假单胞菌群密切相关。表型和化学分类特征进一步证实了它们属于该菌群。基于表型、化学分类特征以及DNA-DNA杂交水平,这三株菌株也可彼此区分,以及与密切相关的东方假单胞菌、布氏假单胞菌和米氏假单胞菌区分开来。因此,建议将菌株CMS 35(T)(=MTCC 4992(T)=DSM 15318(T))、CMS 38(T)(=MTCC 4993(T)=DSM 15319(T))和CMS 64(T)(=MTCC 4994(T)=DSM 15321(T))分别归入假单胞菌属的新物种,即南极假单胞菌(Pseudomonas antarctica sp. nov.)、子午线假单胞菌(Pseudomonas meridiana sp.nov.)和蛋白水解假单胞菌(Pseudomonas proteolytica sp. nov.)。