Kwon Soon Wo, Kim Jong Shik, Park In Cheol, Yoon Sang Hong, Park Duck Hwan, Lim Chun Keun, Go Seung Joo
Korean Agricultural Culture Collection (KACC), Genetic Resources Division, National Institute of Agricultural Biotechnology, 225th, Seodun-dong Gwonseon-gu, Suwon 441-707, Korea.
Korean Agricultural Culture Collection (KACC), Metabolic Engineering Division, National Institute of Agricultural Biotechnology, 225th, Seodun-dong Gwonseon-gu, Suwon 441-707, Korea.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):21-27. doi: 10.1099/ijs.0.02326-0.
Among Pseudomonas strains isolated from Korean agricultural soils, four strains (Ps 9-14 group: Ps 1-2, Ps 1-10, Ps 5-5 and Ps 9-14T) from the Suwon, Goesan and Samchok regions, three strains (Ps 3-10 group: Ps 2-22, Ps 3-1 and Ps 3-10T) from Umsong Region and four strains (Pss 26 group: Pss 14, Pss 25, Pss 26T and Pss 27) from Jinju Region were identified as three independent groups on the basis of 16S rDNA sequence analysis. While, on the basis of 16S rDNA sequence analysis, Ps 9-14T and Ps 3-10T form a phyletic line with Pseudomonas jessenii CIP 105274T, 'Pseudomonas pavonaceae' IAM 1155 and Pseudomonas graminis DSM 11363T, Pss 26T is grouped with Pseudomonas citronellolis ATCC 13674T and Pseudomonas nitroreducens IAM 1439T. According to DNA-DNA hybridization studies, strain Ps 9-14T shows high DNA relatedness to strain Ps 3-10T (52%) and Pseudomonas migulae CIP 105470T (49%) and strain Ps 3-10T reveals high relatedness to strain Ps 9-14T (48%) and P. jessenii CIP 105274T (45%). Strain Pss 26T shows high relatedness to P. citronellolis LMG 18378T (54%), P. nitroreducens ATCC 33634T (48%) and Pseudomonas aeruginosa LMG 1242T (48%). On the basis of phenotypic and genotypic analyses, three novel species of the genus Pseudomonas are proposed: Pseudomonas koreensis sp. nov. (type strain Ps 9-14T =LMG 21318T =KACC 10848T) for the Ps 9-14 group, Pseudomonas umsongensis sp. nov. (type strain Ps 3-10T =LMG 21317T =KACC 10847T) for the Ps 3-10 group and Pseudomonas jinjuensis sp. nov. (type strain Pss 26T =LMG 21316T =KACC 10760T) for the Pss 26 group.
在从韩国农业土壤中分离出的假单胞菌菌株中，通过16S rDNA序列分析，来自水原、槐山和三陟地区的4株菌株（Ps 9 - 14组：Ps 1 - 2、Ps 1 - 10、Ps 5 - 5和Ps 9 - 14T）、来自报恩地区的3株菌株（Ps 3 - 10组：Ps 2 - 22、Ps 3 - 1和Ps 3 - 10T）以及来自晋州地区的4株菌株（Pss 26组：Pss 14、Pss 25、Pss 26T和Pss 27）被鉴定为三个独立的组。同时，基于16S rDNA序列分析，Ps 9 - 14T和Ps 3 - 10T与耶氏假单胞菌CIP 105274T、“孔雀假单胞菌”IAM 1155和禾本科假单胞菌DSM 11363T形成一个进化系，Pss 26T与香茅假单胞菌ATCC 13674T和硝基还原假单胞菌IAM 归为一组。根据DNA - DNA杂交研究，菌株Ps 9 - 14T与菌株Ps 3 - 10T（52%）和迁移假单胞菌CIP 105470T（49%）显示出高度的DNA相关性，菌株Ps 3 - 10T与菌株Ps 9 - 14T（48%）和耶氏假单胞菌CIP 105274T（45%）显示出高度相关性。菌株Pss 26T与香茅假单胞菌LMG 18378T（54%）、硝基还原假单胞菌ATCC 33634T（48%）和铜绿假单胞菌LMG 1242T（48%）显示出高度相关性。基于表型和基因型分析，提出了假单胞菌属的三个新物种：Ps 9 - 14组的韩国假单胞菌新种（模式菌株Ps 9 - 14T =LMG 21318T =KACC 10848T）、Ps 3 - 10组的报恩假单胞菌新种（模式菌株Ps 3 - 10T =LMG 21317T =KACC 10847T）和Pss 26组的晋州假单胞菌新种（模式菌株Pss 26T =LMG 21316T =KACC 10760T）。
