Salvà-Serra Francisco, Nimje Priyank, Piñeiro-Iglesias Beatriz, Alarcón Leonarda Achá, Cardew Sofia, Inganäs Elisabeth, Jensie-Markopoulos Susanne, Ohlén Maria, Sailer Hanna-Sophia, Unosson Christel, Fernández-Juárez Víctor, Pacherres Cesar O, Kühl Michael, Moore Edward R B, Marathe Nachiket P
Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Front Microbiol. 2025 Apr 14;16:1530878. doi: 10.3389/fmicb.2025.1530878. eCollection 2025.
Three β-lactam resistant bacterial strains isolated from gut samples of wild Atlantic mackerel () collected from the northern North Sea were characterized by polyphasic analyses. The strains were determined to belong to the genus but could not be assigned to a known species. The nearly-complete 16S rRNA gene sequence showed the highest similarity (99.9%) to four different species, although partial sequence exhibited relatively low similarities to (93.4%) and other spp. Genome sequencing and subsequent digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) analysis and core genome analysis confirmed that these strains represent a novel species within the genus . The three strains demonstrated ANIb values >99.5% with each other, confirming that all three strains (CCUG 74779 = CECT 30571, CCUG 74780 and CCUG 74781) belong to the same genomospecies. Phylogenomic analysis confirmed that the strains form a distinct genomic clade, representing a novel taxonomic species, for which the name sp. nov., is proposed, with strain CCUG 74779 (=CECT 30571) designated as the type strain. We report the complete genome sequence of the type strain of sp. nov. and show that it carries a gene encoding a novel variant of a chromosomally-encoded class C β-lactamase, which has been designated as PFL-7.
对从北海北部采集的野生大西洋鲭鱼肠道样本中分离出的三株耐β-内酰胺类细菌菌株进行了多相分析。这些菌株被确定属于该属，但无法归入已知物种。近乎完整的16S rRNA基因序列与四个不同物种显示出最高的相似性（99.9%），尽管部分序列与该属（93.4%）和其他该属物种表现出相对较低的相似性。基因组测序以及随后的数字DNA-DNA杂交（dDDH）、平均核苷酸同一性（ANI）分析和核心基因组分析证实，这些菌株代表了该属内的一个新物种。这三株菌株彼此之间的ANIb值>99.5%，证实所有三株菌株（CCUG 74779 = CECT 30571、CCUG 74780和CCUG 74781）属于同一基因组种。系统基因组分析证实，这些菌株形成一个独特的基因组分支，代表一个新的分类物种，为此提出新物种名称“sp. nov.”，并指定菌株CCUG 74779（=CECT 30571）为模式菌株。我们报告了新物种“sp. nov.”模式菌株的完整基因组序列，并表明它携带一个编码染色体编码的C类β-内酰胺酶新变体的基因，该酶已被命名为PFL-7。
