科氏青兰精油在小鼠扭体试验中的镇痛作用。
Antinociceptive effects of the essential oil of Dracocephalum kotschyi in the mouse writhing test.
作者信息
Golshani Samira, Karamkhani Fariba, Monsef-Esfehani Hamid Reza, Abdollahi Mohammad
机构信息
Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Pharm Pharm Sci. 2004 Apr 20;7(1):76-9.
PURPOSE
Dracocephalum kotschyi is a wild-growing flowering plant belonging to the family Labiatae and found abundantly in Iran. This plant has been used in Iran folk medicine as analgesic.
METHODS
The Dracocephalum kotschyi essential oil was isolated and studied on writhing test a visceral pain model in mice. Different constituents of the essential oil were determined by gas chromatography mass spectrophotometry technique.
RESULTS
Limonene, verbenone, alpha-terpineol, perillyl alcohol and caryophyllene were the major constituents of the essential oil. The essential oil in doses (mg kg-1) used 12.5 (13.9%, P<0.05), 25 (43.1%, P<0.01), 50 (68.7%, P<0.01), 75 (39.8%, P<0.01) induced significant reduction in pain response when compared to control. The ED50 was 61.61 mg kg(-1). Hyoscine (1 mg kg(-1)) and indomethacin (5 mg kg(-1)) induced significant (P<0.01) reductions (74.9% and 76.7% respectively) in pain response in comparison to control.
CONCLUSION
This study confirms that antinociceptive properties of D. kotschyi are comparable to those of hyoscine and indomethacin used. Presence of limonene and alpha-terpineol might be responsible for antinociceptive properties of this essential oil. Further studies are necessary to find out a place for it in antispasmodic therapies in human
目的
科氏青兰是一种野生开花植物，属于唇形科，在伊朗大量生长。这种植物在伊朗民间医学中被用作镇痛药。
方法
提取科氏青兰精油，并在小鼠内脏疼痛模型扭体试验中进行研究。采用气相色谱 - 质谱技术测定精油的不同成分。
结果
柠檬烯、马鞭草烯酮、α - 松油醇、紫苏醇和石竹烯是精油的主要成分。与对照组相比，所用剂量（mg/kg）为12.5（13.9%，P<0.05）、25（43.1%，P<0.01）、50（68.7%，P<0.01）、75（39.8%，P<0.01）的精油能显著降低疼痛反应。半数有效剂量（ED50）为61.61mg/kg。与对照组相比，东莨菪碱（1mg/kg）和吲哚美辛（5mg/kg）能显著（P<0.01）降低疼痛反应（分别为74.9%和76.7%）。
结论
本研究证实科氏青兰的镇痛特性与所用的东莨菪碱和吲哚美辛相当。柠檬烯和α - 松油醇的存在可能是这种精油具有镇痛特性的原因。有必要进一步研究以确定其在人类解痉治疗中的地位。
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