Moradi Hossein, Ghavam Mansureh, Tavili Ali
Department of Range and Watershed Management, Faculty of Natural Resources and Earth Sciences, University of Kashan, Kashan, Iran.
Department of Rehabilitation of Arid and Mountainous, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
Biotechnol Rep (Amst). 2019 Dec 3;25:e00408. doi: 10.1016/j.btre.2019.e00408. eCollection 2020 Mar.
Boiss is one of eight species of , an endemic species of Iran, and grows in areas such as Isfahan, Yasuj, Mazandaran, and Tabriz. The present study was designed to analyze the antioxidant, phenol and flavonoids contents of this plant under different conditions of cultivation in a completely randomized, factorial design.
Shrubs of different ages (two, three, and six) in cultivated rangeland collected from three randomly plant at the end of May 2018 simultaneously with the flowering time. Antioxidant activity of methanolic extract of plant samples were evaluated with DPPH method. Total phenolic compounds and the total content of flavonoids were measured using the Folin Sioukhlu and aluminum chloride methods respectively.
The result showed that there was a significant effect of planting time, plant organs and interaction of time and organ on the total antioxidant capacity and total phenol and flavonoids contents. The highest antioxidant activity, total phenol, and total flavonoid have belonged to the flower of six age plants. The highest antioxidant activity, total phenol, and total flavonoid have belonged to the flower of six age. The highest level of antioxidant activity with IC50 15.8489 ± 0.001 μg/ml belonged to the flower of the two-year-old plant, which has a stronger antioxidant activity than the BHT standard with 19.95 μg/ml.
But in general, it can be said that the six-year-old plant with the highest antioxidant capacity, phenolic, and flavonoid compounds is the best age and mainly flower organ is preferred.
博伊斯（Boiss）是伊朗特有的八种植物之一，生长在伊斯法罕、亚苏季、马赞德兰和大不里士等地。本研究旨在采用完全随机析因设计，分析该植物在不同栽培条件下的抗氧化剂、酚类和黄酮类含量。
2018年5月底花期时，从三个随机种植地收集不同年龄（两年、三年和六年）的栽培牧场灌木。用DPPH法评估植物样品甲醇提取物的抗氧化活性。分别用福林-肖卡鲁法和氯化铝法测定总酚化合物和黄酮类总含量。
结果表明，种植时间、植物器官以及时间与器官的相互作用对总抗氧化能力、总酚和黄酮类含量有显著影响。抗氧化活性、总酚和总黄酮含量最高的属于六年生植物的花。抗氧化活性最高、总酚和总黄酮含量最高的属于六年生植物的花。抗氧化活性最高水平（IC50为15.8489±0.001μg/ml）属于两年生植物的花，其抗氧化活性比标准丁基羟基甲苯（BHT）（19.95μg/ml）更强。
但总体而言，可以说抗氧化能力、酚类和黄酮类化合物含量最高的六年生植物是最佳年龄，且主要优选花器官。
