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溶纤维丁酸弧菌tet(W)基因位于新型接合转座子TnB1230上,该转座子包含重复的硝基还原酶编码序列。

The Butyrivibrio fibrisolvens tet(W) gene is carried on the novel conjugative transposon TnB1230, which contains duplicated nitroreductase coding sequences.

作者信息

Melville Claire M, Brunel Romain, Flint Harry J, Scott Karen P

机构信息

Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom.

出版信息

J Bacteriol. 2004 Jun;186(11):3656-9. doi: 10.1128/JB.186.11.3656-3659.2004.

Abstract

The Butyrivibrio fibrisolvens tet(W) gene is located on the conjugative transposon TnB1230. TnB1230 encodes transfer proteins with 48 to 67% identity to some of those encoded by Tn1549. tet(W) is flanked by directly repeated sequences with significant homology to oxygen-insensitive nitroreductases. The 340 nucleotides upstream of tet(W) are strongly conserved and are required for tetracycline resistance.

摘要

溶纤维丁酸弧菌tet(W)基因位于接合转座子TnB1230上。TnB1230编码的转移蛋白与Tn1549编码的某些转移蛋白具有48%至67%的同一性。tet(W)两侧是与氧不敏感硝基还原酶具有显著同源性的直接重复序列。tet(W)上游的340个核苷酸高度保守,是四环素抗性所必需的。

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