Kwiecień Ewelina, Stefańska Ilona, Chrobak-Chmiel Dorota, Kizerwetter-Świda Magdalena, Moroz Agata, Olech Wanda, Spinu Marina, Binek Marian, Rzewuska Magdalena
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8 St., 02-786 Warsaw, Poland.
Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c St., 02-786 Warsaw, Poland.
Antibiotics (Basel). 2021 Apr 3;10(4):380. doi: 10.3390/antibiotics10040380.
Determinants of tetracycline resistance in are still poorly known. In this study, resistance to tetracycline was investigated in 114 isolates from livestock and European bison. Tetracycline minimum inhibitory concentration (MIC) was evaluated by a microdilution method, and tetracycline resistance genes were detected by PCR. To determine variants of and their linkage with mobile elements, sequencing analysis was performed. Among the studied isolates, 43.0% were tetracycline resistant (MIC ≥ 8 µg/mL). The highest MIC of tetracycline (32 µg/mL) was noted in bovine and European bison isolates. The most prevalent determinant of tetracycline resistance was (in 40.4% of isolates), while was detected only in 8.8% of isolates. Four variants of (, , , ) were recognized. The variant was the most frequent and was linked to the ATE-1 transposon. The 2 variant, found in a swine isolate, was not previously reported in . This is the first report on determinants of tetracycline resistance in isolates from European bison. These findings highlight that wild animals, including wild ruminants not treated with antimicrobials, can be a reservoir of tetracycline-resistant bacteria carrying resistance determinants, which may be easily spread among pathogenic and environmental microorganisms.
目前，关于[具体物种名称未给出]中四环素抗性的决定因素仍知之甚少。在本研究中，对来自家畜和欧洲野牛的114株[具体物种名称未给出]分离株的四环素抗性进行了调查。采用微量稀释法评估四环素最低抑菌浓度（MIC），并通过PCR检测四环素抗性基因。为了确定[具体物种名称未给出]的变体及其与移动元件的连锁关系，进行了测序分析。在所研究的分离株中，43.0%对四环素具有抗性（MIC≥8μg/mL）。在牛和欧洲野牛分离株中观察到四环素的最高MIC（32μg/mL）。四环素抗性最普遍的决定因素是[具体基因名称未给出]（在40.4%的分离株中），而[另一种具体基因名称未给出]仅在8.8%的分离株中检测到。识别出[具体基因名称未给出]的四种变体（[变体1名称未给出]、[变体2名称未给出]、[变体3名称未给出]、[变体4名称未给出]）。[变体1名称未给出]变体最为常见，并与ATE - 1转座子相关。在一株猪分离株中发现的[变体2名称未给出]变体，此前在[具体物种名称未给出]中未见报道。这是关于欧洲野牛[具体物种名称未给出]分离株中四环素抗性决定因素的首次报告。这些发现突出表明，包括未接受抗菌药物治疗的野生反刍动物在内的野生动物，可能是携带抗性决定因素的四环素抗性细菌的储存库，这些细菌可能很容易在致病微生物和环境微生物之间传播。
