鲑鱼立克次氏体在昆虫组织培养细胞中生长至高滴度。
Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells.
作者信息
Birkbeck T Harry, Griffen Angela A, Reid Helen I, Laidler L Anthony, Wadsworth Simon
机构信息
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
出版信息
Infect Immun. 2004 Jun;72(6):3693-4. doi: 10.1128/IAI.72.6.3693-3694.2004.
Abstract
Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.
摘要
鲑鱼立克次氏体在已建立的昆虫、青蛙和鱼类组织培养细胞中生长。鲑鱼立克次氏体在Sf21细胞中的产量比在CHSE - 214细胞中获得的产量高100倍，并且对大西洋鲑鱼的毒力得以保留。鲑鱼立克次氏体在Sf21细胞中生长的最高温度为24摄氏度。
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