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Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR.通过聚合酶链式反应（PCR）对养殖鲑科鱼类中的鲑立克次氏体进行微创检测。
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Rickettsioses as paradigms of new or emerging infectious diseases.立克次体病作为新发或新兴传染病的范例。
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Rickettsia-like organism in farmed salmon.
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Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.从南非爪蟾（非洲爪蟾）建立细胞系（XTC - 2）。
Experientia. 1973 Apr 15;29(4):466-7. doi: 10.1007/BF01926785.
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Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes.鲑鱼立克次氏体属，新属，新种，鲑科鱼类一种流行性疾病的病原体。
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10
The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).从昆虫草地贪夜蛾（鳞翅目；夜蛾科）建立了两个细胞系。
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鲑鱼立克次氏体在昆虫组织培养细胞中生长至高滴度。

Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells.

作者信息

Birkbeck T Harry, Griffen Angela A, Reid Helen I, Laidler L Anthony, Wadsworth Simon

机构信息

Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.

出版信息

Infect Immun. 2004 Jun;72(6):3693-4. doi: 10.1128/IAI.72.6.3693-3694.2004.

DOI:10.1128/IAI.72.6.3693-3694.2004
PMID:15155687
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC415715/
Abstract

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.

摘要

鲑鱼立克次氏体在已建立的昆虫、青蛙和鱼类组织培养细胞中生长。鲑鱼立克次氏体在Sf21细胞中的产量比在CHSE - 214细胞中获得的产量高100倍，并且对大西洋鲑鱼的毒力得以保留。鲑鱼立克次氏体在Sf21细胞中生长的最高温度为24摄氏度。