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Appl Environ Microbiol. 1998 Aug;64(8):3066-9. doi: 10.1128/AEM.64.8.3066-3069.1998.
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本文引用的文献

1
The rickettsia: an emerging group of pathogens in fish.立克次氏体：鱼类中一种新出现的病原体群体。
Emerg Infect Dis. 1997 Apr-Jun;3(2):137-44. doi: 10.3201/eid0302.970206.
2
In vitro infection of a cell line from Ictalurus nebulosus with Piscirickettsia salmonis.用鲑鱼立克次氏体对来自斑点叉尾鮰的细胞系进行体外感染。
Can J Vet Res. 1997 Jan;61(1):66-8.
3
Antigenic characterization of the salmonid pathogen Piscirickettsia salmonis.鲑科鱼类病原体鲑鱼立克次氏体的抗原特性
Infect Immun. 1996 Dec;64(12):5205-10. doi: 10.1128/iai.64.12.5205-5210.1996.
4
Real time quantitative PCR.实时定量聚合酶链反应
Genome Res. 1996 Oct;6(10):986-94. doi: 10.1101/gr.6.10.986.
5
Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments.通过16S rDNA片段的变性梯度凝胶电泳比较两个加利福尼亚河口浮游细菌的系统发育组成。
Appl Environ Microbiol. 1996 Jul;62(7):2676-80. doi: 10.1128/aem.62.7.2676-2680.1996.
6
Solid-phase reversible immobilization for the isolation of PCR products.用于分离PCR产物的固相可逆固定化。
Nucleic Acids Res. 1995 Nov 25;23(22):4742-3. doi: 10.1093/nar/23.22.4742.
7
Fish cell lines: establishment and characterization of nine cell lines from salmonids.鱼类细胞系：九种鲑科鱼类细胞系的建立与特性分析
In Vitro. 1984 Sep;20(9):671-6. doi: 10.1007/BF02618871.
8
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.Chelex 100作为一种从法医材料中简单提取用于基于PCR分型的DNA的介质。
Biotechniques. 1991 Apr;10(4):506-13.
9
Maximizing sensitivity and specificity of PCR by pre-amplification heating.通过预扩增加热最大化聚合酶链反应的灵敏度和特异性。
Nucleic Acids Res. 1991 Jul 11;19(13):3749. doi: 10.1093/nar/19.13.3749.
10
Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes.鲑鱼立克次氏体属，新属，新种，鲑科鱼类一种流行性疾病的病原体。
Int J Syst Bacteriol. 1992 Jan;42(1):120-6. doi: 10.1099/00207713-42-1-120.

通过聚合酶链式反应（PCR）对养殖鲑科鱼类中的鲑立克次氏体进行微创检测。

Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR.

作者信息

Marshall S, Heath S, Henríquez V, Orrego C

机构信息

Instituto de Biología, Universidad Católica de Valparaíso, Valparaíso, Chile.

出版信息

Appl Environ Microbiol. 1998 Aug;64(8):3066-9. doi: 10.1128/AEM.64.8.3066-3069.1998.

DOI:10.1128/AEM.64.8.3066-3069.1998
PMID:9687475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106817/
Abstract

The attributes of the PCR allowed implementation of an assay for specific detection of Piscirickettsia salmonis from a few microliters of fish serum. This opens the way to less invasive modes of sampling for this microbial pathogen in salmonids.

摘要

聚合酶链反应（PCR）的特性使得能够从几微升鱼血清中实施对鲑鱼立克次氏体进行特异性检测的分析方法。这为在鲑科鱼类中对这种微生物病原体采用侵入性较小的采样方式开辟了道路。