微流控设备在合成测序法中可读取多达四个连续的碱基对。
Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis.
作者信息
Kartalov Emil P, Quake Stephen R
机构信息
Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
Nucleic Acids Res. 2004 May 20;32(9):2873-9. doi: 10.1093/nar/gkh613. Print 2004.
Abstract
We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.
摘要
我们开发了首个用于合成测序的全集成微流控系统。利用该芯片和荧光检测，我们已成功可靠地对多达4个连续碱基对进行了测序。所描述的测序仪可与同一芯片上的其他微流控组件集成，以实现真正的芯片实验室技术。设计用于将DNA固定在弹性微通道上的表面化学在广泛的研究和应用中都很有用。
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