Papaparaskevas Joseph, Houhoula Dimitra P, Papadimitriou Maria, Saroglou Georgios, Legakis Nicholas J, Zerva Loukia
National and Kapodistrian University of Athens Medical School, Athens, Greece.
Emerg Infect Dis. 2004 Apr;10(4):732-5. doi: 10.3201/eid1004.030544.
Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anthracis-selective agar.
排除炭疽芽孢杆菌方法的优化可提高产量、缩短周转时间并减轻工作量。我们使用72株环境中非炭疽芽孢杆菌的杆菌来验证排除炭疽芽孢杆菌的方法。最有效的方法是使用马血琼脂、将分离株在胰蛋白酶大豆肉汤中培养2小时后进行动力试验,以及用炭疽芽孢杆菌选择性琼脂筛选分离株。