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肉鸡日粮中微生物植酸酶的评估。

Evaluation of microbial phytase in broiler diets.

作者信息

Dilger R N, Onyango E M, Sands J S, Adeola O

机构信息

Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907-1151, USA.

出版信息

Poult Sci. 2004 Jun;83(6):962-70. doi: 10.1093/ps/83.6.962.

Abstract

Two trials were conducted to evaluate the efficacy of a new microbial phytase (Phyzyme XP) for broiler chicks. Trial 1 used 192 8-d-old male broilers in a 14-d trial to assess growth and nutrient utilization. Dietary treatments (221.9 g/kg CP) included a positive control [5.0 g/kg nonphytate P (NPP)], negative control (1.2 g/kg NPP), and negative control plus 500 or 1,000 phytase units/kg of diet. Phytase addition increased weight gain, feed intake, feed efficiency, and tibia and toe ash (linear, P < 0.01) with tibia ash also responding quadratically (P < 0.05). Apparent ileal digestibility of P (linear and quadratic, P < 0.05), tryptophan, and valine (linear, P < 0.05) also increased. Linear and quadratic responses were observed for retention of DM, nitrogen, P, and several amino acids (P < 0.05) with added phytase. Trial 2 utilized 576 1-d-old male broilers over a 42-d period to evaluate growth performance. Diets were formulated for starter (222.7 g/kg CP) and grower (201.5 g/kg CP) phases and included a positive control (starter and grower, 5.0 and 3.8 g/kg NPP, respectively); negative control (starter and grower, 2.4 and 1.8 g/kg NPP, respectively); negative control plus 500, 750, or 1,000 phytase units/kg; and negative control plus 500 phytase units/kg of Natuphos phytase. Phytase increased weight gain and feed intake (starter, grower, overall) as well as feed efficiency during the starter period (linear, P < 0.05). Feed intake was also improved during the grower period and overall (quadratic, P < 0.05). Tibia and toe ash of birds fed for the first 21 d increased (linear, P < 0.05) with tibia ash also increasing quadratically (P < 0.05). Overall, tibia and toe ash were improved due to phytase addition (linear and quadratic, P < 0.05). In conclusion, this microbial phytase, derived from Escherichia coli and expressed in Schizosaccaromyces pombe, elicited improved growth performance, bone mineralization, and P utilization in broiler chicks.

摘要

进行了两项试验以评估一种新型微生物植酸酶(Phyzyme XP)对肉仔鸡的功效。试验1使用192只8日龄雄性肉仔鸡,进行为期14天的试验,以评估生长和养分利用率。日粮处理(粗蛋白含量221.9 g/kg)包括一个阳性对照[非植酸磷(NPP)含量5.0 g/kg]、阴性对照(NPP含量1.2 g/kg)以及阴性对照加500或1000植酸酶单位/kg日粮。添加植酸酶提高了体重增加、采食量、饲料效率以及胫骨和脚趾骨灰分(呈线性,P<0.01),胫骨骨灰分也呈二次曲线响应(P<0.05)。磷(呈线性和二次曲线,P<0.05)、色氨酸和缬氨酸(呈线性,P<0.05)的回肠表观消化率也有所提高。添加植酸酶后,干物质、氮、磷和几种氨基酸的保留呈线性和二次曲线响应(P<0.05)。试验2在42天的时间里使用576只1日龄雄性肉仔鸡来评估生长性能。日粮针对雏鸡期(粗蛋白含量222.7 g/kg)和生长鸡期(粗蛋白含量201.5 g/kg)进行配制,包括一个阳性对照(雏鸡期和生长鸡期分别为5.0和3.8 g/kg NPP);阴性对照(雏鸡期和生长鸡期分别为2.4和1.8 g/kg NPP);阴性对照加500、750或1000植酸酶单位/kg;以及阴性对照加500植酸酶单位/kg的Natuphos植酸酶。植酸酶提高了体重增加和采食量(雏鸡期、生长鸡期、总体)以及雏鸡期的饲料效率(呈线性,P<\0.05)。生长鸡期和总体的采食量也有所改善(呈二次曲线,P<0.05)。在前21天饲喂的鸡的胫骨和脚趾骨灰分增加(呈线性,P<0.05),胫骨骨灰分也呈二次曲线增加(P<0.05)。总体而言,添加植酸酶改善了胫骨和脚趾骨灰分(呈线性和二次曲线,P<0.05)。总之,这种源自大肠杆菌并在粟酒裂殖酵母中表达的微生物植酸酶提高了肉仔鸡的生长性能、骨骼矿化和磷利用率。

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