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康复病例血清中抗体所识别的SARS冠状病毒肽段。

SARS corona virus peptides recognized by antibodies in the sera of convalescent cases.

作者信息

Guo Jian-Ping, Petric Martin, Campbell William, McGeer Patrick L

机构信息

Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.

出版信息

Virology. 2004 Jul 1;324(2):251-6. doi: 10.1016/j.virol.2004.04.017.

DOI:10.1016/j.virol.2004.04.017
PMID:15207612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7125913/
Abstract

We synthesized on cellulose membranes 4942 ten-amino-acid peptides which included all of the sequences predicted for the severe acute respiratory syndrome (SARS) corona virus. We probed these membranes with four pairs of acute and convalescent sera from recovered SARS cases. We correlated positively reacting peptides with the in vitro SARS-CoV neutralizing activity of the samples. We found that convalescent sera with high neutralizing activity recognized exclusively only a limited number of peptides on the membranes. This suggests that antibodies against the epitopes represented by these peptides could be responsible for much of the SARS-CoV neutralizing activity. The findings have implications for monitoring humoral responses to SARS-CoV as well as for developing a successful SARS vaccine.

摘要

我们在纤维素膜上合成了4942种十肽氨基酸肽，这些肽包含了严重急性呼吸综合征（SARS）冠状病毒预测的所有序列。我们用来自康复SARS病例的四对急性期和恢复期血清检测这些膜。我们将呈阳性反应的肽与样品的体外SARS-CoV中和活性相关联。我们发现，具有高中和活性的恢复期血清仅能识别膜上有限数量的肽。这表明，针对这些肽所代表的表位的抗体可能是SARS-CoV中和活性的主要原因。这些发现对于监测对SARS-CoV的体液反应以及开发成功的SARS疫苗具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3aa/7125913/fac5861a081b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3aa/7125913/5c63ce691d02/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3aa/7125913/fac5861a081b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3aa/7125913/5c63ce691d02/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3aa/7125913/fac5861a081b/gr2.jpg

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SARS corona virus peptides recognized by antibodies in the sera of convalescent cases.

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[6]
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[9]
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[10]
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本文引用的文献

[1]
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus.

Nature. 2003-11-27

[2]
Virology: SARS virus infection of cats and ferrets.

Nature. 2003-10-30

[3]
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Vaccine. 2003-10-1

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J Mol Biol. 2003-8-29

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Lancet. 2003-5-24

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Science. 2003-5-30

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Science. 2003-5-30

[8]
Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation.

J Virol. 2001-10

[9]
Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier.

J Virol. 2000-2

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