Wollensak Gregor, Spörl Eberhard, Pham Duy-Thoai
Department of Ophthalmology, Technical University of Dresden, Dresden, Germany.
J Cataract Refract Surg. 2004 Jul;30(7):1526-30. doi: 10.1016/j.jcrs.2003.11.031.
To examine the effect of trypan blue staining on the biomechanical behavior of the porcine anterior lens capsule.
Department of Ophthalmology, Technical University of Dresden, Dresden, Germany.
Fifty-five anterior lens capsules from porcine cadaver eyes were used. Two parallel 8.0 mm x 4.0 mm large capsule strips were prepared from each capsule. After trypan blue staining for various time intervals combined with exposure to white light (6000 lux) or with no light exposure, biomechanical stress-strain measurements were performed using an automated material tester. Untreated specimens and specimens treated with glutaraldehyde 0.1% were used as controls. The absorption spectrum of trypan blue 0.1% solution and the emission spectrum of the light source were measured.
After treatment with light and trypan blue, at 25% strain, there was a statistically significant increase in stress of up to 70.1% and in elastic stiffness of 47% and a decrease in the ultimate mechanical strain of up to 13%. There were no biomechanical changes in capsules with trypan blue staining in the absence of light or after a short illumination time of 30 seconds, indicating a light-dependent process. After 30 minutes of glutaraldehyde 0.1% treatment, there was an increase in stress of 321.6% at 25% strain and a decrease in the ultimate strain of 47.6%. The emission spectrum of the light source included the absorption peak for trypan blue at 580 nm.
Trypan blue staining of the lens capsule combined with light irradiation for at least 1 minute led to an increase in elastic stiffness at 25% strain and a reduction in the ultimate extensibility. This effect is probably due to the photosensitizing action of trypan blue, leading to light-induced collagen crosslinking of the capsule collagen similar to age-related crosslinking. Nucleus expression might be impeded by the increased capsule stiffness. Continuous curvilinear capsulorhexis is facilitated.
研究台盼蓝染色对猪晶状体前囊生物力学行为的影响。
德国德累斯顿工业大学眼科。
使用来自猪尸体眼的55个晶状体前囊。从每个囊中制备两条平行的8.0毫米×4.0毫米大的囊带。在台盼蓝染色不同时间间隔后，结合白光照射（6000勒克斯）或不进行光照，使用自动材料测试仪进行生物力学应力-应变测量。未处理的标本和用0.1%戊二醛处理的标本用作对照。测量0.1%台盼蓝溶液的吸收光谱和光源的发射光谱。
光照和台盼蓝处理后，在25%应变时，应力显著增加高达70.1%，弹性刚度增加47%，极限机械应变降低高达13%。在无光条件下或短时间光照30秒后台盼蓝染色的囊中没有生物力学变化，表明这是一个光依赖过程。0.1%戊二醛处理30分钟后，在25%应变时应力增加321.6%，极限应变降低了47.6%。光源的发射光谱包括台盼蓝在580纳米处的吸收峰。
晶状体囊台盼蓝染色并结合至少1分钟的光照导致在25%应变时弹性刚度增加，极限伸展性降低。这种效应可能是由于台盼蓝的光敏作用，导致囊胶原的光诱导胶原交联，类似于与年龄相关的交联。核表达可能会因囊刚度增加而受到阻碍。连续曲线撕囊更容易进行。
