[血红蛋白变性试验作为替代Draize眼刺激试验的应用研究]

[Study on the use of haemoglobin denaturation test as an alternative to Draize eye irritation test].

作者信息

Liao Yan, Wang Xue, Zhang Li-shi, Li Guan-min, Zhang Bao-xu

机构信息

Department of Natrition and Food Hygience, West China School of Public Health, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):654-7.

PMID:15460411

Abstract

OBJECTIVE

To use haemoglobin denaturation test (HD test) as an alternative to Draize eye irritation test (Draize test).

METHODS

Fourteen cosmetic ingredients were tested by HD test. The results were compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and Score of 24 h after application (S24).

RESULTS

The correlation coefficient between RDC50 and MAS and that between RDC50 and S24 were 0.926 and 0.921 respectively, while that between 1%lambdamax and MAS, and between 1%lambdamax and S24 were 0.881 and 0.791 respectively. The results showed that RDC50 had a higher correlation with Draize test than 1%lambdamax did, but in the use of RDC50 some information of data would be lost. On the other hand, 1%lambdamax, which had a greater correlation with corneal score in the three component scores of the Draize test, could be used for assessing water-insoluble chemicals.

CONCLUSION

The results showed that HD test could be used as an effective alternative to Draize eye irritation test.

摘要

目的

使用血红蛋白变性试验（HD试验）替代兔眼刺激试验（Draize试验）。

方法

采用HD试验对14种化妆品成分进行检测。将结果与Draize试验中的两种评分，即最大平均Draize总分（MAS）和用药后24小时评分（S24）进行比较。

结果

RDC50与MAS之间的相关系数以及RDC50与S24之间的相关系数分别为0.926和0.921，而1%λmax与MAS之间的相关系数以及1%λmax与S24之间的相关系数分别为0.881和0.791。结果表明，RDC50与Draize试验的相关性高于1%λmax，但使用RDC50时会丢失一些数据信息。另一方面，1%λmax与Draize试验三个成分评分中的角膜评分相关性更大，可用于评估水不溶性化学物质。