Leiro J, Arranz J A, Paramá A, Alvarez M F, Sanmartín M L
Laboratorio de Parasitología, Instituto de Investigación y Análisis Alimentarios, Universidad de Santiago de Compostela, Constantino Candeira 15782, Santiago de Compostela, Spain.
Dis Aquat Organ. 2004 May 5;59(2):171-4. doi: 10.3354/dao059171.
This study investigated the in vitro effects of the polyphenols resveratrol, mangiferin and (-)-epigallocatechin-3-gallate (EGCG) on the histiophagous ciliate Philasterides dicentrarchi, which causes fatal scuticociliatosis in farmed turbot Scophthalmus maximus L. Of the 3 polyphenols, resveratrol showed strongest antiprotozoal activity, reducing ciliate density after 1 wk culture by, on average, 91% at 50 microM, and 96% at 500 microM. EGCG reduced ciliate density by, on average, 93% at 500 microM, with no significant effect at 50 microM. Mangiferin reduced ciliate density by, on average, 56% at 500 microM, again with no significant effect at 50 microM. In view of these findings, we discuss the potential utility of chemotherapy with polyphenols as a strategy for the control of scuticociliatosis in farmed turbot.
本研究调查了多酚白藜芦醇、芒果苷和（-）-表没食子儿茶素-3-没食子酸酯（EGCG）对噬菌纤毛虫双盘藻（Philasterides dicentrarchi）的体外作用，该纤毛虫可在养殖大菱鲆（Scophthalmus maximus L.）中引发致命的盾纤毛虫病。在这3种多酚中，白藜芦醇表现出最强的抗原生动物活性，在50微摩尔浓度下培养1周后，纤毛虫密度平均降低91%，在500微摩尔浓度下降低96%。EGCG在500微摩尔浓度下使纤毛虫密度平均降低93%，在50微摩尔浓度下无显著影响。芒果苷在500微摩尔浓度下使纤毛虫密度平均降低56%，同样在50微摩尔浓度下无显著影响。鉴于这些发现，我们讨论了用多酚进行化学疗法作为控制养殖大菱鲆盾纤毛虫病策略的潜在效用。
