Borbat Peter P, Davis Jared H, Butcher Samuel E, Freed Jack H
Department of Chemistry and Chemical Biology, B52 Baker Lab, Cornell University, Ithaca, New York 14853, USA.
J Am Chem Soc. 2004 Jun 30;126(25):7746-7. doi: 10.1021/ja049372o.
It is shown how the new technique of double-quantum filtered refocused electron spin-echoes is a significant improvement over double-quantum coherence ESR, since it increases the experimental acquisition time. This enables the measurement of longer distances in bilabeled biomolecules. The method is demonstrated on a long double-stranded A-type RNA, spin labeled at both ends. The measured distance of 72 A is in excellent agreement with molecular modeling.
