变形链球菌NG5分选酶A细胞壁锚定活性丧失的点突变鉴定
Identification of a point mutation resulting in loss of cell wall anchoring activity of SrtA of Streptococcus mutans NG5.
作者信息
Lee Song F, McGavin Mary K H
机构信息
Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5.
出版信息
Infect Immun. 2004 Jul;72(7):4314-7. doi: 10.1128/IAI.72.7.4314-4317.2004.
Abstract
Streptococcus mutans NG5 failed to anchor antigen P1 to the cell surface, and such a failure could be attributed to a defective SrtA, which was made defective by a point mutation within the srtA gene. Without a functional SrtA, S. mutans NG5 was not able to perform a number of cell surface-related activities, including saliva-mediated adherence and aggregation.
摘要
变形链球菌NG5无法将抗原P1锚定到细胞表面，这种失败可能归因于分选酶A（SrtA）缺陷，该缺陷是由srtA基因内的点突变造成的。由于没有功能性的分选酶A，变形链球菌NG5无法进行许多与细胞表面相关的活动，包括唾液介导的黏附和聚集。
相似文献
1
Identification of a point mutation resulting in loss of cell wall anchoring activity of SrtA of Streptococcus mutans NG5.变形链球菌NG5分选酶A细胞壁锚定活性丧失的点突变鉴定
Infect Immun. 2004 Jul;72(7):4314-7. doi: 10.1128/IAI.72.7.4314-4317.2004.
2
Roles of sortase in surface expression of the major protein adhesin P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans.分选酶在变形链球菌主要蛋白黏附素P1的表面表达、唾液诱导的聚集与黏附以及致龋性中的作用。
Infect Immun. 2003 Feb;71(2):676-81. doi: 10.1128/IAI.71.2.676-681.2003.
3
The sortase of Streptococcus mutans mediates cell wall anchoring of a surface protein antigen.变形链球菌的分选酶介导一种表面蛋白抗原的细胞壁锚定。
Oral Microbiol Immunol. 2003 Aug;18(4):266-9. doi: 10.1034/j.1399-302x.2003.00076.x.
4
Inactivation of srtA gene of Streptococcus mutans inhibits dextran-dependent aggregation by glucan-binding protein C.变形链球菌srtA基因的失活可抑制葡聚糖结合蛋白C介导的依赖葡聚糖的聚集。
Oral Microbiol Immunol. 2004 Feb;19(1):57-60. doi: 10.1046/j.0902-0055.2003.00104.x.
5
Effect of SrtA on Interspecies Adherence of Oral Bacteria.SrtA 对口腔细菌种间黏附的影响。
Curr Med Sci. 2018 Feb;38(1):160-166. doi: 10.1007/s11596-018-1860-y. Epub 2018 Mar 15.
6
Astilbin Inhibits the Activity of Sortase A from .梓醇抑制 . 菌株的 sortase A 活性。
Molecules. 2019 Jan 28;24(3):465. doi: 10.3390/molecules24030465.
7
Roles of Streptococcus mutans dextranase anchored to the cell wall by sortase.变形链球菌葡聚糖酶通过分选酶锚定在细胞壁上的作用。
Oral Microbiol Immunol. 2004 Apr;19(2):102-5. doi: 10.1046/j.0902-0055.2003.00123.x.
8
Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans.变形链球菌分选酶A基因错义突变对酶活性的影响。
BMC Oral Health. 2016 Apr 11;16:47. doi: 10.1186/s12903-016-0204-1.
9
Deletion in sortase gene of Streptococcus mutans Ingbritt.变形链球菌英布里特株分选酶基因的缺失
Oral Microbiol Immunol. 2004 Jun;19(3):210-3. doi: 10.1111/j.0902-0055.2004.00136.x.
10
Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case-control study.儿童龋齿相关分选酶A基因的遗传多态性及社会行为因素：一项病例对照研究
BMC Oral Health. 2015 May 2;15:54. doi: 10.1186/s12903-015-0039-1.
引用本文的文献
1
Genotypic diversity of Streptococcus mutans in children with and without early childhood caries- A systematic review.患与未患幼儿龋的儿童中变形链球菌的基因型多样性——一项系统评价
J Oral Biol Craniofac Res. 2021 Apr-Jun;11(2):308-312. doi: 10.1016/j.jobcr.2021.03.002. Epub 2021 Mar 14.
2
Diversity of SpaP in genetic and salivary agglutinin mediated adherence among Streptococcus mutans strains.变形链球菌 SpaP 在遗传和唾液黏附素介导黏附中的多样性。
Sci Rep. 2019 Dec 27;9(1):19943. doi: 10.1038/s41598-019-56486-9.
3
[Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis].变形链球菌分选酶A功能与致龋性之间的联系：一项初步代谢组学分析
Hua Xi Kou Qiang Yi Xue Za Zhi. 2018 Aug 1;36(4):360-366. doi: 10.7518/hxkq.2018.04.003.
4
Effect of SrtA on Interspecies Adherence of Oral Bacteria.SrtA 对口腔细菌种间黏附的影响。
Curr Med Sci. 2018 Feb;38(1):160-166. doi: 10.1007/s11596-018-1860-y. Epub 2018 Mar 15.
5
In silico identification of potential inhibitors targeting Streptococcus mutans sortase A.计算机筛选靶向变异链球菌表面蛋白 A 转肽酶的潜在抑制剂
Int J Oral Sci. 2017 Mar;9(1):53-62. doi: 10.1038/ijos.2016.58.
6
Effects of missense mutations in sortase A gene on enzyme activity in Streptococcus mutans.变形链球菌分选酶A基因错义突变对酶活性的影响。
BMC Oral Health. 2016 Apr 11;16:47. doi: 10.1186/s12903-016-0204-1.
7
Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case-control study.儿童龋齿相关分选酶A基因的遗传多态性及社会行为因素：一项病例对照研究
BMC Oral Health. 2015 May 2;15:54. doi: 10.1186/s12903-015-0039-1.
8
Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.分选酶与将蛋白质锚定到革兰氏阳性菌包膜上的技术。
Microbiol Mol Biol Rev. 2006 Mar;70(1):192-221. doi: 10.1128/MMBR.70.1.192-221.2006.
本文引用的文献
1
Roles of sortase in surface expression of the major protein adhesin P1, saliva-induced aggregation and adherence, and cariogenicity of Streptococcus mutans.分选酶在变形链球菌主要蛋白黏附素P1的表面表达、唾液诱导的聚集与黏附以及致龋性中的作用。
Infect Immun. 2003 Feb;71(2):676-81. doi: 10.1128/IAI.71.2.676-681.2003.
2
Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence.单核细胞增生李斯特菌中srtA基因的失活会抑制表面蛋白的锚定并影响毒力。
Mol Microbiol. 2002 Feb;43(4):869-81. doi: 10.1046/j.1365-2958.2002.02798.x.
3
The sortase SrtA of Listeria monocytogenes is involved in processing of internalin and in virulence.单核细胞增生李斯特菌的分选酶SrtA参与内化素的加工及毒力形成。
Infect Immun. 2002 Mar;70(3):1382-90. doi: 10.1128/IAI.70.3.1382-1390.2002.
4
Inactivation of the srtA gene in Streptococcus gordonii inhibits cell wall anchoring of surface proteins and decreases in vitro and in vivo adhesion.戈登链球菌中srtA基因的失活会抑制表面蛋白的细胞壁锚定，并降低其在体外和体内的黏附能力。
Infect Immun. 2001 Jan;69(1):75-80. doi: 10.1128/IAI.69.1.75-80.2001.
5
6
Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections.金黄色葡萄球菌分选酶突变体在表面蛋白展示和动物感染发病机制方面存在缺陷。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5510-5. doi: 10.1073/pnas.080520697.
7
Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif.分选酶的纯化与特性研究，分选酶是一种在LPXTG基序处切割金黄色葡萄球菌表面蛋白的转肽酶。
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12424-9. doi: 10.1073/pnas.96.22.12424.
8
Subcellular localization of the Streptococcus mutans P1 protein C terminus.
Can J Microbiol. 1999 Jun;45(6):536-9.
9
Virulence of a spaP mutant of Streptococcus mutans in a gnotobiotic rat model.变形链球菌spaP突变体在悉生大鼠模型中的毒力
Infect Immun. 1999 Mar;67(3):1201-6. doi: 10.1128/IAI.67.3.1201-1206.1999.
10
Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans GS-5.变形链球菌GS-5的PAc抗原中一个移码突变的鉴定，该突变导致提前终止并丧失细胞壁锚定功能。
Infect Immun. 1997 Feb;65(2):794-7. doi: 10.1128/iai.65.2.794-797.1997.
Zotero 插件