变形链球菌GS-5的PAc抗原中一个移码突变的鉴定，该突变导致提前终止并丧失细胞壁锚定功能。
Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans GS-5.
作者信息
Murakami Y, Nakano Y, Yamashita Y, Koga T
机构信息
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
出版信息
Infect Immun. 1997 Feb;65(2):794-7. doi: 10.1128/iai.65.2.794-797.1997.
Abstract
Most strains of Streptococcus mutans possess a 190-kDa protein antigen (PAc) on their cell surfaces, while strain GS-5 produces extracellularly a 155-kDa PAc protein. The pac gene of strain GS-5 consists of 3,477 bp and codes for a protein of 1,158 amino acids. One insertion of an adenine into the 3,469th, 3,470th, or 3,471st position from the start codon results in a frameshift mutation at codon 1157 with subsequent termination after 3 additional codons.
摘要
大多数变形链球菌菌株在其细胞表面具有一种190 kDa的蛋白质抗原（PAc），而GS-5菌株在细胞外产生一种155 kDa的PAc蛋白。GS-5菌株的pac基因由3477个碱基对组成，编码一个1158个氨基酸的蛋白质。从起始密码子开始，在第3469、3470或3471位插入一个腺嘌呤会导致密码子1157处的移码突变，随后在另外3个密码子后终止。
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