Kordower J H, Piecinski P, Rakic P
Department of Neurological Sciences, Rush Presbyterian/St. Lukes Medical Center, Chicago, IL 60612.
Brain Res Dev Brain Res. 1992 Jul 24;68(1):9-15. doi: 10.1016/0165-3806(92)90242-o.
The time course of neurogenesis for neurons which comprise the amygdaloid complex in Rhesus monkeys was determined using tritiated thymidine autoradiography. Fourteen pregnant monkeys received injections of tritiated thymidine between embryonic days 27 (E27) and 56 of their 165 day gestation and offspring were sacrificed during the early postnatal period. The first neurons destined for the amygdaloid complex were generated at E33 making them among the earliest postmitotic neurons in the telencephalon. Neurogenesis peaked within all nuclei of the amygdaloid complex between E38 and E48 and had ceased between E50 and E56. While amygdaloid neurogenesis in postnatally sacrificed monkeys displayed a dorsal-to-ventral gradient of radiolabeled neurons, the considerable rotation of the temporal lobe during the latter stages of primate development indicates that neurogenesis in the embryo, during the first third of gestation, actually occurs across a medial-to-lateral gradient. This medial-to-lateral gradient occurs as a smooth wave across the amygdaloid nuclei and does not respect neuroanatomical subdivisions or patterns of connectivity of the amygdaloid nuclei in the Rhesus monkey.
利用氚标记胸腺嘧啶核苷放射自显影术确定了恒河猴杏仁复合体中神经元的神经发生时间进程。14只怀孕的猴子在妊娠165天中的胚胎第27天（E27）至第56天期间接受了氚标记胸腺嘧啶核苷注射，并在出生后早期处死后代。最早注定要进入杏仁复合体的神经元在E33产生，使其成为端脑中最早的有丝分裂后神经元之一。神经发生在E38至E48期间在杏仁复合体的所有核内达到峰值，并在E50至E56之间停止。虽然在出生后处死的猴子中杏仁核神经发生显示出放射性标记神经元的背腹梯度，但在灵长类动物发育后期颞叶的显著旋转表明，在妊娠前三分之一期间胚胎中的神经发生实际上是沿着中侧梯度发生的。这种中侧梯度以平滑波的形式穿过杏仁核，并且不遵循恒河猴杏仁核的神经解剖学细分或连接模式。
