白藜芦醇对微小孢子虫兔脑炎微孢子虫活力和感染性的体外作用
In vitro effects of resveratrol on the viability and infectivity of the microsporidian Encephalitozoon cuniculi.
作者信息
Leiro José, Cano Ernesto, Ubeira Florencio M, Orallo Francisco, Sanmartín Manuel L
机构信息
Laboratorio de Parasitología, Instituto de Investigación y Análisis Alimentarios, Universidad de Santiago de Compostela, C/Constantino Candeira s/n, 15782 Santiago de Compostela, Spain.
出版信息
Antimicrob Agents Chemother. 2004 Jul;48(7):2497-501. doi: 10.1128/AAC.48.7.2497-2501.2004.
Abstract
Microsporidians of the genus Encephalitozoon are an important cause of disease in immunocompromised patients, and there are currently no completely effective treatments. The present study investigated the viability and infectivity of spores of Encephalitozoon cuniculi that had been exposed to resveratrol (RESV), a natural phytoalexin found in grapes and red wine. RESV at 50 microM showed significant sporicidal activity, and at 10 to 50 microM it reduced the capacity of the spores to infect dog kidney epithelial cells of the MDCK line. At 10 microM RESV also significantly inhibited intracellular development of the parasite, without affecting host cell viability. These results suggest that RESV may be useful in the treatment of Encephalitozoon infections.
摘要
脑胞内原虫属的微孢子虫是免疫功能低下患者疾病的重要病因，目前尚无完全有效的治疗方法。本研究调查了经白藜芦醇（RESV，一种存在于葡萄和红酒中的天然植保素）处理的兔脑炎微孢子虫孢子的活力和感染性。50微摩尔的RESV显示出显著的杀孢子活性，在10至50微摩尔时，它降低了孢子感染MDCK系犬肾上皮细胞的能力。在10微摩尔时，RESV还显著抑制了寄生虫的细胞内发育，而不影响宿主细胞的活力。这些结果表明，RESV可能对治疗脑胞内原虫感染有用。
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