通过改良的美国国立临床实验室标准化委员会（NCCLS）方法和基于活性的2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[(苯胺基)羰基]-2H-氢氧化四唑（XTT）测定法评估马杜拉足分支菌对伊曲康唑和两性霉素B的体外敏感性。
In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay.
作者信息
Ahmed Abdalla O A, van de Sande Wendy W J, van Vianen Wim, van Belkum Alex, Fahal Ahmed H, Verbrugh Henri A, Bakker-Woudenberg Irma A J M
机构信息
Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
出版信息
Antimicrob Agents Chemother. 2004 Jul;48(7):2742-6. doi: 10.1128/AAC.48.7.2742-2746.2004.
Abstract
Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC(50)]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC(50) of 0.5 to 1.0 mg/liter).
摘要
根据美国国家临床实验室标准委员会（NCCLS）指南的方案（目视读数）以及基于2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[(苯胺基)羰基]-2H-氢氧化四唑（XTT）的真菌活力测定法，测定了马杜拉足分支菌对两性霉素B和伊曲康唑的体外敏感性。XTT测定法对这两种抗真菌药物均具有可重复性和敏感性。伊曲康唑（抑制50%受试分离株的最低抑菌浓度[MIC(50)]为0.06至0.13毫克/升）优于两性霉素B（MIC(50)为0.5至1.0毫克/升）。
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