高浓度孕酮可诱导顶体反应，且细胞毒性作用较低。

High progesterone concentrations induce acrosome reaction with a low cytotoxic effect.

作者信息

Parinaud J, Labal B, Vieitez G

机构信息

Institut National de la Santé et de la Recherche Médicale, (INSERM CJF 89-08), Toulouse, France.

出版信息

Fertil Steril. 1992 Sep;58(3):599-602. doi: 10.1016/s0015-0282(16)55270-4.

DOI:10.1016/s0015-0282(16)55270-4

PMID:1521658

Abstract

OBJECTIVE

To determine the optimal conditions to obtain live acrosome-reacted spermatozoa for micromanipulation.

DESIGN

Experiments were performed to determine time and dose-dependent effects of calcium ionophore A23187 or steroids on acrosome reaction of fertile donor sperm. The percentages of total reacted and live reacted spermatozoa were assessed with the peanut agglutinin lectin procedure.

RESULTS

Incubation with 1 mmol/L progesterone (P) induced 48% +/- 17% acrosome reaction after 6 hours. Motility and viability remained high (49% +/- 3% and 70% +/- 2%, respectively) and thus the percentage of live reacted spermatozoa was 27% +/- 5%. Incubation with A23187 (5 mumol/L for 30 minutes) gave similar results for the percentage of live reacted spermatozoa (26% +/- 4%) but with a lower motility and viability (25% +/- 7% and 53% +/- 2%, respectively; P less than 0.05).

CONCLUSIONS

These results show that high concentration of P is an effective way to induce acrosome reaction in preparation for micromanipulation.

摘要

目的

确定获得用于显微操作的活的顶体反应精子的最佳条件。

设计

进行实验以确定钙离子载体A23187或类固醇对可育供体精子顶体反应的时间和剂量依赖性影响。用花生凝集素程序评估总反应精子和活的反应精子的百分比。

结果

用1 mmol/L孕酮（P）孵育6小时后诱导48%±17%的顶体反应。活力和生存力仍较高（分别为49%±3%和70%±2%），因此活的反应精子的百分比为27%±5%。用A23187（5 μmol/L，30分钟）孵育，活的反应精子的百分比结果相似（26%±4%），但活力和生存力较低（分别为25%±7%和53%±2%；P<0.05）。