Duailibi M T, Duailibi S E, Young C S, Bartlett J D, Vacanti J P, Yelick P C
University Federal of São Paulo, Department of Otorhinolaryngology and Human Communication Disorders, Sao Paolo, Brazil.
J Dent Res. 2004 Jul;83(7):523-8. doi: 10.1177/154405910408300703.
The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
近期利用猪牙胚组织进行复杂牙齿结构的生物工程研究表明，哺乳动物牙齿组织再生具有潜力。我们通过比较从出生后3至7天（dpn）的大鼠获取的培养牙胚细胞在牙齿组织工程应用中的效用，改进了牙齿生物工程方法。将接种细胞的可生物降解支架在成年大鼠宿主的网膜中培养12周，然后收获。对12周植入组织的分析表明，解离的4-dpn大鼠牙胚细胞在PGA或PLGA支架上接种1小时，能最可靠地生成生物工程牙齿组织。我们得出结论，牙齿组织工程方法可用于生成猪和大鼠的牙齿组织。此外，我们从培养的牙胚细胞生物工程构建牙齿结构的能力表明，牙齿上皮和间充质干细胞可在体外维持至少6天。
