Bryndorf T, Kirchhoff M, Larsen J, Andreasson B, Bjerregaard B, Westh H, Rose H, Lundsteen C
Fertility Clinic, Rigshospitalet, Denmark.
Cytogenet Genome Res. 2004;106(1):43-8. doi: 10.1159/000078559.
We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. All samples were also human papilloma virus (HPV)-genotyped. Gain of chromosome 1, the aberration most often seen in VIN III (67%), was not seen in HPV-positive or -negative VSCCs (0%). Both VIN III and VSCC frequently showed gain of 3q (56 and 70%, respectively). The VIN III samples often demonstrated gain of 20q (56%) and 20p (44%), and the VSCC samples gain of 8q (60%), loss of 3p (50%), and 8p (40%). None of the four most frequent changes in the VSCC samples occurred exclusively in the HPV-positive or -negative samples. As expected, we did not find any cytogenetic changes in condylomas and nearly any changes in VIN I-II.
我们通过高分辨率比较基因组杂交（HR-CGH）和流式细胞术分析了尖锐湿疣（4例）、外阴上皮内瘤变I-III级（VIN I-III，11例）以及原发性外阴鳞状细胞癌（VSCC，10例）的基因变化。所有样本也进行了人乳头瘤病毒（HPV）基因分型。1号染色体的增加是VIN III中最常出现的畸变（67%），但在HPV阳性或阴性的VSCC中均未出现（0%）。VIN III和VSCC均经常出现3q增加（分别为56%和70%）。VIN III样本常表现出20q增加（56%）和20p增加（44%），VSCC样本则表现出8q增加（60%）、3p缺失（50%）和8p缺失（40%）。VSCC样本中最常见的四种变化均未仅出现在HPV阳性或阴性样本中。正如预期的那样，我们在尖锐湿疣中未发现任何细胞遗传学变化，在VIN I-II中几乎也未发现任何变化。
