Leite Sônia Pereira, de Medeiros Paloma Lys, da Silva Eliete Cavalcanti, de Souza Maia Maria Bernadete, de Menezes Lima Vera Lúcia, Saul Daniel Eduardo
Laboratório de Cultura de Células II, Departamento de Histologia e Embriologia do Centro de Ciências Biológicas da, Universidade Federal de Pernambuco (UFPE), Cidade Universitária, Recife 50, 670-420 Pernambuco, Brazil.
Reprod Toxicol. 2004 Jul;18(5):701-5. doi: 10.1016/j.reprotox.2004.04.004.
Aqueous extract of leaves of Indigofera suffruticosa (AELIs) were studied for adverse effects in preimplantation mouse embryos. Two-cell mouse embryos were cultured for 94 h in human tubal fluid medium (HTF), and AELIs at a concentration of 5 or 10 mg/ml. On Day 4 of culture, morulae and blastocysts were collected for morphological analysis of blastomeres. We found that embryos exposed to the higher concentration of AELIs (10 mg/ml) did not develop and all embryos persisted at the two-cell stage. Those embryos exposed to the lower concentration (5 mg/ml) showed development until morula, blastocyst and hatched blastocyst stages that were similar to the controls. These results suggest that use of AELIs may be hazardous to humans who make use of it in folk medicine.
对灌木状木蓝叶子的水提取物（AELIs）在植入前小鼠胚胎中的不良反应进行了研究。将二细胞期小鼠胚胎在人输卵管液培养基（HTF）中培养94小时，并添加浓度为5或10毫克/毫升的AELIs。在培养的第4天，收集桑椹胚和囊胚用于卵裂球的形态分析。我们发现，暴露于较高浓度AELIs（10毫克/毫升）的胚胎没有发育，所有胚胎都停滞在二细胞期。那些暴露于较低浓度（5毫克/毫升）的胚胎显示出能够发育到桑椹胚、囊胚和孵化囊胚阶段，与对照组相似。这些结果表明，使用AELIs可能对在民间医学中使用它的人有危害。
