Rodrigues Natalie Emanuelle Ribeiro, Oliveira Alisson Rodrigo da Silva, Lima Sandrine Maria de Arruda, Nunes Daniel Medeiros, Albuquerque Priscilla Barbosa Sales de, da Cunha Maria das Graças Carneiro, Wanderley Almir Gonçalves, Júnior Flavio Manoel Rodrigues da Silva, Silva José Bruno Nunes Ferreira, Teixeira Álvaro Aguiar Coelho, Silva Teresinha Gonçalves da
Laboratory of Farmatoxicological Prospecting of Bioactive Products (BIOFARMATOX), Department of Antibiotics, Federal University of Pernambuco (UFPE), Recife 54740-520, Pernambuco, Brazil.
Department of Medicine, University of Pernambuco (UPE), Garanhuns 53294-902, Pernambuco, Brazil.
Curr Issues Mol Biol. 2023 Sep 19;45(9):7617-7629. doi: 10.3390/cimb45090479.
Toxicological studies on medicinal plants are essential to ensure their safety and effectiveness in treating various diseases. Despite the species L. being popularly used in the treatment of several diseases due to the pharmacological properties of its bioactive compounds, there are few studies in the literature regarding its toxicity regarding reproduction. Therefore, the purpose of this study was to assess the potential embryotoxic and teratogenic effects of the aqueous extract of leaves (AECi) on Wistar rats. Animals were given AECi at doses of 100, 200, and 400 mg/kg during the pre-implantation and organogenesis periods. Data were analyzed using ANOVA followed by Tukey's test and Kruskal-Wallis. Pregnant rats treated during the pre-implantation period showed no signs of reproductive toxicity. Rats that received AECi at 100, 200, and 400 mg/kg during organogenesis did not exhibit any signs of maternal systemic toxicity or significant differences in gestational and embryotoxic parameters. Some skeletal changes were observed in the treated groups. Therefore, it can be suggested that AECi at doses of 100, 200, and 400 mg/kg is safe for treated animals and does not induce reproductive toxicity under the experimental conditions applied, but it also caused low systemic toxicity.
对药用植物进行毒理学研究对于确保其在治疗各种疾病中的安全性和有效性至关重要。尽管由于其生物活性化合物的药理特性，该植物物种被广泛用于治疗多种疾病，但文献中关于其生殖毒性的研究却很少。因此，本研究的目的是评估叶水提取物（AECi）对Wistar大鼠的潜在胚胎毒性和致畸作用。在植入前和器官形成期，给动物分别给予100、200和400mg/kg剂量的AECi。数据采用方差分析，随后进行Tukey检验和Kruskal-Wallis检验。在植入前期接受治疗的怀孕大鼠未表现出生殖毒性迹象。在器官形成期接受100、200和400mg/kg AECi的大鼠未表现出任何母体全身毒性迹象，在妊娠和胚胎毒性参数方面也没有显著差异。在治疗组中观察到了一些骨骼变化。因此，可以认为，在本实验条件下，100、200和400mg/kg剂量的AECi对受试动物是安全的，不会引起生殖毒性，但也会导致低全身毒性。
