分析电离辐射后的细胞周期检查点。

Analyzing cell cycle checkpoints after ionizing radiation.

作者信息

Xu Bo, Kastan Michael B

机构信息

Department of Genetics and Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, USA.

出版信息

Methods Mol Biol. 2004;281:283-92. doi: 10.1385/1-59259-811-0:283.

DOI:10.1385/1-59259-811-0:283

PMID:15220537

Abstract

Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.

摘要

本章介绍了几种用于测量暴露于电离辐射后细胞周期扰动的方法。这些方法包括通过5'-溴脱氧尿苷（BrdUrd）标记进行G1期检查点测定，随后进行流式细胞术分析；通过测量对电离辐射的DNA合成来进行S期检查点测定；以及通过定量电离辐射后组蛋白H3磷酸化来进行G2/M期检查点测定。