Shinozaki Kuniko, Okada Mineyuki, Nagashima Shigeo, Kaiho Ikuo, Taniguchi Koki
Division of Virology, Chiba Prefectural Institute of Public Health, Chiba, Japan.
J Med Virol. 2004 Aug;73(4):612-6. doi: 10.1002/jmv.20134.
Two G12 human rotavirus strains, CP727 and CP1030, were isolated from the respective diarrheic stools of an infant and an adult in Japan. VP7 gene sequences of strains CP727 and CP1030 showed high identity with that of the G12 prototype strain L26, and with those of G12 strains reported recently from Thailand, the United States, and India. VP4 gene sequences of strains CP727 and CP1030 showed the highest identity with those of P[9] rotaviruses. In Northern blot hybridization, strains CP727 and CP1030 were found to be closely related to strain AU-1 (G3P[9]); nine RNA segments hybridized to each other. Moreover, all segments each of the two Japanese G12 strains hybridized to those of the Thai G12 strain T152. These results suggest that Japanese G12 strains detected in this study are reassortants between a L26-like strain and a strain in the AU-1 genogroup. A similar reassortant was found in the Thai G12 strain T152.
从日本一名婴儿和一名成人的腹泻粪便中分别分离出两株G12人轮状病毒，CP727和CP1030。CP727和CP1030毒株的VP7基因序列与G12原型毒株L26以及最近从泰国、美国和印度报道的G12毒株的序列高度一致。CP727和CP1030毒株的VP4基因序列与P[9]轮状病毒的序列一致性最高。在Northern印迹杂交中，发现CP727和CP1030毒株与AU-1毒株（G3P[9]）密切相关；九个RNA片段相互杂交。此外，两株日本G12毒株的所有片段均与泰国G12毒株T152的片段杂交。这些结果表明，本研究中检测到的日本G12毒株是L26样毒株与AU-1基因组中一个毒株之间的重配体。在泰国G12毒株T152中也发现了类似的重配体。
