Kmita Hanna, Antos Nina, Wojtkowska Malgorzata, Hryniewiecka Lilla
Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701 Poznan, Poland.
J Bioenerg Biomembr. 2004 Apr;36(2):187-93. doi: 10.1023/b:jobb.0000023622.66554.a6.
It has been shown recently that in Saccharomyces cerevisiae mitochondria depleted of the VDAC channel (delta por1 mitochondria), the TOM complex channel substitutes for the VDAC channel. The additional function of the TOM complex channel is probably facilitated by the upregulation of nuclear-encoded components of the TOM complex as has been shown for Tom40 (a major component of the channel) and Tom70 (one of the surface receptors). Here we report that in S. cerevisiae cells the VDAC channel seems to be an important signal in the expression of the TOM complex components. S. cerevisiae cells depleted of the VDAC channel (delta por1 cells) contain distinctly increased levels of Tom40mRNA, and Tom70mRNA, but their synthesis and translation are affected differentially by the applied inhibitors of transcription and translation. Consequently, it may be concluded that depletion of the VDAC channel might influence differentially the expression of TOM40 and TOM70 genes.
最近研究表明，在酿酒酵母中，缺乏电压依赖性阴离子通道（VDAC通道）的线粒体（δpor1线粒体）中，转位酶外膜复合物（TOM复合物）通道可替代VDAC通道。正如Tom40（通道的主要成分）和Tom70（表面受体之一）所示，TOM复合物通道的额外功能可能是由TOM复合物的核编码成分上调所促进的。在此我们报告，在酿酒酵母细胞中，VDAC通道似乎是TOM复合物成分表达中的一个重要信号。缺乏VDAC通道的酿酒酵母细胞（δpor1细胞）中Tom40mRNA和Tom70mRNA水平明显升高，但它们的合成和翻译受到所应用的转录和翻译抑制剂的不同影响。因此，可以得出结论，VDAC通道的缺失可能对TOM40和TOM70基因的表达产生不同影响。
