Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland.
J Bioenerg Biomembr. 2010 Dec;42(6):483-9. doi: 10.1007/s10863-010-9315-6. Epub 2010 Nov 12.
Available data suggest that voltage-dependent anion selective channel (VDAC) constitutes an important component of a cellular regulatory mechanism based on the intracellular reduction/oxidation (redox) state. Here, using quantitative RT-PCR, we demonstrated that depletion of VDAC1 (termed here VDAC) in Saccharomyces cerevisiae cells distinctly affected levels of mRNAs encoding nuclear proteins sensitive to changes of the intracellular redox state including the nuclear transcription factors important for adaptation to the redox state and proteins involved in communication between mitochondria and the nucleus. We also revealed that the changes of the studied protein transcript levels generally correlated with changes of the intracellular redox state although VDAC appears also to affect mRNA levels by a mechanism not based on changes of the intracellular redox states. Thus, VDAC seems to be an important element of the intracellular signaling network.
现有数据表明，电压依赖性阴离子选择通道（VDAC）构成了基于细胞内还原/氧化（redox）状态的细胞调节机制的重要组成部分。在这里，我们使用定量 RT-PCR 证明了酿酒酵母细胞中 VDAC1（此处称为 VDAC）的耗竭明显影响了编码对细胞内 redox 状态变化敏感的核蛋白的 mRNA 水平，包括对 redox 状态适应很重要的核转录因子和参与线粒体与核之间通讯的蛋白质。我们还揭示了所研究的蛋白质转录本水平的变化通常与细胞内 redox 状态的变化相关，尽管 VDAC 似乎也通过一种不基于细胞内 redox 状态变化的机制影响 mRNA 水平。因此，VDAC 似乎是细胞内信号网络的重要组成部分。
