根据巴西近期的抗菌药物敏感性概况分析脆弱拟杆菌菌株耐药性的决定因素。
Determinants of resistance in Bacteroides fragilis strains according to recent Brazilian profiles of antimicrobial susceptibility.
作者信息
Paula G R, Falcão L S, Antunes E N F, Avelar K E S, Reis F N A, Maluhy M A, Ferreira M C S, Domingues R M C P
机构信息
Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão 21941-590, Rio de Janeiro, RJ, Brazil.
出版信息
Int J Antimicrob Agents. 2004 Jul;24(1):53-8. doi: 10.1016/j.ijantimicag.2003.11.011.
Susceptibility profiles of 99 Bacteroides fragilis strains for 9 antimicrobial agents were defined by using an agar dilution method. The isolates were uniformly susceptible to imipenen and metronidazole. All isolates were resistant to ampicillin. The resistance rates to amoxicillin/clavulanate, cefoxitin, cefotaxime, chloramphenicol, clindamycin and tetracycline were 3.0, 12.1, 15.1, 1.0, 18.2 and 75.7%, respectively. Sixteen strains showed reduced susceptibility to metronidazole (MIC 2-4 mg/L) but none had nim genes using PCR. All strains were also investigated for the presence of cepA, cfiA, cfxA, ermF and tetQ genes by PCR methodology and 92.9, 4.9, 24.2, 2 and 64.6% of the strains were respectively found positive. These results reflect the importance of surveys of susceptibility profiles and the relevance of detecting major genetic determinants to monitor the dissemination of these genes.
采用琼脂稀释法确定了99株脆弱拟杆菌对9种抗菌药物的敏感性谱。分离株对亚胺培南和甲硝唑均敏感。所有分离株对氨苄西林耐药。对阿莫西林/克拉维酸、头孢西丁、头孢噻肟、氯霉素、克林霉素和四环素的耐药率分别为3.0%、12.1%、15.1%、1.0%、18.2%和75.7%。16株菌株对甲硝唑的敏感性降低（MIC为2 - 4mg/L），但通过PCR检测均未发现nim基因。采用PCR方法对所有菌株的cepA、cfiA、cfxA、ermF和tetQ基因进行检测，分别有92.9%、4.9%、24.2%、2%和64.6%的菌株呈阳性。这些结果反映了敏感性谱调查的重要性以及检测主要遗传决定因素对监测这些基因传播的相关性。
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