气相色谱法测定尿中2,5-己二酮作为正己烷暴露的指标。
Gas chromatographic determination of 2,5-hexanedione in urine as an indicator of exposure to n-hexane.
作者信息
Vohra K, Gaind V S
机构信息
Occupational Health Laboratory, Ontario Ministry of Labour, Weston, Canada.
出版信息
J Anal Toxicol. 1992 May-Jun;16(3):176-8. doi: 10.1093/jat/16.3.176.
A sensitive and specific method for determination of 2,5-hexanedione in urine is described. Treatment of the urine specimen directly with n-butylamine yields n-butyl 2,5-dimethyl pyrrole. The latter is extracted into diisopropylether and analyzed by gas chromatography (GC) with flame ionization detection (FID), thermionic specific detection (TSD) (N mode), or mass spectrometric detection (MS). The minimum detectable quantities are 1 mg/L urine when employing FID with a coefficient of variation of less than 6%. Recovery of 2,5-hexanedione added to the urine at the level of 10 mg/L was 78.9%.
本文描述了一种灵敏且特异的测定尿中2,5 -己二酮的方法。尿样直接用正丁胺处理后生成2,5 -二甲基吡咯正丁酯。后者用二异丙醚萃取，并用带有火焰离子化检测（FID）、热离子特异性检测（TSD）（N模式）或质谱检测（MS）的气相色谱（GC）进行分析。采用FID时，最低检测量为1 mg/L尿样，变异系数小于6%。向尿样中添加10 mg/L的2,5 -己二酮时，回收率为78.9%。
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