人型支原体精氨酸脱亚氨酶基因的核苷酸序列。

Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis.

作者信息

Harasawa R, Koshimizu K, Kitagawa M, Asada K, Kato I

机构信息

Faculty of Medicine, University of Tokyo, Japan.

出版信息

Microbiol Immunol. 1992;36(6):661-5. doi: 10.1111/j.1348-0421.1992.tb02068.x.

DOI:10.1111/j.1348-0421.1992.tb02068.x

PMID:1522817

Abstract

The arginine deiminase gene of Mycoplasma hominis was amplified by the polymerase chain reaction, and its entire nucleotide sequence was determined. This gene consists of 1227 base pairs encoding 409 amino acids, and has 35.2% guanine plus cytosine content. Nucleotide sequence homologies of the arginine deiminase gene between M. hominis and M. arginini, and between M. hominis and M. orale were 82.1 and 80.8%, respectively, suggesting that this gene is highly conserved among arginine-utilizing Mycoplasma species.

摘要

通过聚合酶链反应扩增人型支原体的精氨酸脱亚氨酶基因，并测定其完整的核苷酸序列。该基因由1227个碱基对组成，编码409个氨基酸，鸟嘌呤加胞嘧啶含量为35.2%。人型支原体与精氨酸支原体以及人型支原体与口腔支原体之间精氨酸脱亚氨酶基因的核苷酸序列同源性分别为82.1%和80.8%，这表明该基因在利用精氨酸的支原体物种中高度保守。