Ohno T, Ando O, Sugimura K, Taniai M, Suzuki M, Fukuda S, Nagase Y, Yamamoto K, Azuma I
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Infect Immun. 1990 Nov;58(11):3788-95. doi: 10.1128/iai.58.11.3788-3795.1990.
The existence of a mycoplasmal arginine deiminase which catalyzes the conversion of L-arginine to L-citrulline has been postulated. Here we show the partial amino acid sequence of arginine deiminase of Mycoplasma arginini and the complete nucleotide sequence of the arginine deiminase gene of M. arginini. The open reading frame deduced from this sequence consists of 1,230 bp encoding 410 amino acids. The mature form of this enzyme contains 409 amino acids after the deletion of the first methionine. In this open reading frame, TGA nonsense codons are used as tryptophan codons; this usage was verified by determination of the amino acid sequence. The molecular weight of the enzyme calculated from the deduced amino acid sequence is 46,372. Recently, the nucleotide sequence of the arginine deiminase gene of M. arginini was reported by Kondo et al. (K. Kondo, H. Sone, H. Yoshida, T. Toida, K. Kanatani, Y.-M. Hong N. Nishino, and J. Tanaka, Mol. Gen. Genet. 221:81-86, 1990). However, their sequence differed from ours in several places and especially at the C terminus.
有人推测支原体中存在一种精氨酸脱亚氨酶，它可催化L-精氨酸转化为L-瓜氨酸。在此我们展示了精氨酸支原体精氨酸脱亚氨酶的部分氨基酸序列以及精氨酸支原体精氨酸脱亚氨酶基因的完整核苷酸序列。从该序列推导的开放阅读框由1230个碱基对组成，编码410个氨基酸。该酶的成熟形式在缺失第一个甲硫氨酸后含有409个氨基酸。在这个开放阅读框中，TGA无义密码子被用作色氨酸密码子；这种用法通过氨基酸序列测定得到了验证。根据推导的氨基酸序列计算，该酶的分子量为46372。最近，近藤等人报道了精氨酸支原体精氨酸脱亚氨酶基因的核苷酸序列（近藤K、索尼H、吉田H、户井田T、金谷K、洪Y - M、西野N和田中J，《分子与普通遗传学》221:81 - 86，1990）。然而，他们的序列在几个地方与我们的不同，尤其是在C末端。
