Sugimura K, Ohno T, Azuma I, Yamamoto K
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Infect Immun. 1993 Jan;61(1):329-31. doi: 10.1128/iai.61.1.329-331.1993.
The extent of restriction fragment length polymorphism in genes for the arginine deiminase enzyme among 28 species of mycoplasmas was assessed by Southern blot analysis of DNA digested with EcoRI or TaqI nuclease probed with a 725-bp internal fragment of the arginine deiminase gene from Mycoplasma arginini. The results indicated unexpected heterogeneity among species of a single genus.
通过用EcoRI或TaqI核酸酶消化DNA，并用来自精氨酸支原体的精氨酸脱亚氨酶基因的725bp内部片段进行探针杂交，对28种支原体中精氨酸脱亚氨酶基因的限制性片段长度多态性程度进行了Southern印迹分析。结果表明，同一属的不同种之间存在意想不到的异质性。
