毒蛛毒素和哇巴因对大鼠大脑皮质切片乙酰胆碱释放及胞质和粗囊泡组分乙酰胆碱含量的影响。

Effects of tityustoxin and ouabain on release of acetylcholine from slices of cortex from the rat brain and on the acetylcholine content of cytoplasmic and crude vesicular fractions.

作者信息

Prado M A, Silva M A, Moraes-Santos T, Gomez M V

机构信息

Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas UFMG, Belo Horizonte, Minas Gerais, Brazil.

出版信息

Neuropharmacology. 1992 Apr;31(4):383-7. doi: 10.1016/0028-3908(92)90071-v.

DOI:10.1016/0028-3908(92)90071-v

PMID:1522956

Abstract

In this paper, the effects of tityustoxin and ouabain on the release and mobilization of ACh from cytoplasmic and vesicular stores of slices of cortex from the brain of the rat are described. Tityustoxin induced an increase of release of ACh which was time-dependent and this effect was accompanied by a concomitant decrease of the ACh, measured in cytoplasmic and crude vesicular fractions. Unlike tityustoxin, ouabain did not increase the release and synthesis of ACh during a 2 min period of stimulation but later, at 5, 10 and 15 min, ouabain increased the release and synthesis of ACh from incubated slices of cortex. After 10 min, ouabain caused a reduction of the content of ACh from cytoplasmic and crude vesicular fractions.

摘要

本文描述了美洲毒蛛毒素和哇巴因对大鼠大脑皮层切片细胞质和囊泡储存中乙酰胆碱释放和动员的影响。美洲毒蛛毒素诱导乙酰胆碱释放增加，且这种效应具有时间依赖性，同时在细胞质和粗囊泡部分测得的乙酰胆碱含量相应减少。与美洲毒蛛毒素不同，哇巴因在2分钟的刺激期内并未增加乙酰胆碱的释放和合成，但在随后的5、10和15分钟时，哇巴因增加了孵育的皮层切片中乙酰胆碱的释放和合成。10分钟后，哇巴因导致细胞质和粗囊泡部分的乙酰胆碱含量降低。