蝎毒——替尤斯毒素对大鼠脑皮质切片高亲和力胆碱摄取的影响。

The effect of the scorpion venom, tityustoxin, on high-affinity choline uptake in rat brain cortical slices.

作者信息

Macedo T M, Gomez M V

出版信息

Neuropharmacology. 1983 Feb;22(2):233-7. doi: 10.1016/0028-3908(83)90014-x.

Abstract

Tityustoxin (TsTx) inhibited high affinity choline uptake (HAChU) in cortical slices of the rat brain. The effect was dependent on the concentration of tityustoxin, energy source, incubation time, temperature, and the pH of the incubation medium. The inhibitory effect was dependent upon the presence of sodium and calcium ions in the incubation medium; barium ions could not replace calcium. Both tetrodotoxin and ethyleneglycol-tetra-acetic acid (EGTA) blocked the inhibitory effect of tityustoxin on high affinity choline uptake. On this evidence, it is suggested that the effect of tityustoxin might be related to its action on cell depolarization, causing an increase in the release of acetylcholine (ACh).

摘要

巴西游走蛛毒素（TsTx）抑制大鼠大脑皮层切片中的高亲和力胆碱摄取（HAChU）。该效应取决于巴西游走蛛毒素的浓度、能量来源、孵育时间、温度以及孵育培养基的pH值。抑制效应取决于孵育培养基中钠和钙离子的存在；钡离子不能替代钙离子。河豚毒素和乙二醇四乙酸（EGTA）均能阻断巴西游走蛛毒素对高亲和力胆碱摄取的抑制作用。基于这些证据，有人提出巴西游走蛛毒素的作用可能与其对细胞去极化的作用有关，从而导致乙酰胆碱（ACh）释放增加。