蝎毒中蒂氏毒素（TsTX）对脑片乙酰胆碱释放和合成的影响。

Effects of tityustoxin (TsTX) from scorpion venom on the release and synthesis of acetylcholine in brain slices.

作者信息

Macedo T M, Gomez M V

出版信息

Toxicon. 1982;20(3):601-6. doi: 10.1016/0041-0101(82)90054-x.

Abstract

Tityustoxin (TsTX) increased the release and synthesis of acetylcholine (ACh) in slices of rat brain hippocampus, hypothalamus, thalamus, cortex and striatum. The effect was highest in slices of hippocampus and cortex and smallest in hypothalamus. These effects of TsTX were dependent on the presence of Na+ and Ca2+ in the incubation medium. EGTA, 0.1 mM, blocked the effect of TsTX in all areas, except for the hippocampus and frontal striatum, where a concentration of 10 mM was required. Tetrodotoxin blocked the increase in the release and synthesis of ACh induced by tityustoxin.

摘要

巴西游走蛛毒素（TsTX）可增加大鼠脑海马、下丘脑、丘脑、皮层和纹状体切片中乙酰胆碱（ACh）的释放和合成。在海马和皮层切片中的作用最强，在下丘脑切片中的作用最弱。TsTX的这些作用取决于孵育培养基中Na+和Ca2+的存在。0.1 mM的乙二醇双（2-氨基乙基醚）四乙酸（EGTA）可阻断TsTX在所有区域的作用，但海马和额叶纹状体除外，在这两个区域需要10 mM的浓度。河豚毒素可阻断巴西游走蛛毒素诱导的ACh释放和合成增加。