Feng J, Somlyo A V, Somlyo A P
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908, USA.
J Microsc. 2004 Jul;215(Pt 1):92-9. doi: 10.1111/j.0022-2720.2004.01342.x.
A compositional imaging system based on simultaneous scanning electron energy-loss spectroscopy (EELS) and energy-dispersive X-ray spectroscopy (EDS) was developed. This system utilizes the combined power of EELS and EDS for quantitative compositional imaging at nanometre resolution. The system is particularly suitable for, but not limited to, biological research, as it simultaneously provides sensitive maps of an element such as Ca or P from EELS and of many other elements from EDS. Degradation of resolution by specimen drift is prevented by correcting for drift during data acquisition, using image cross-correlation. Several advanced features are implemented for real-time and/or off-line quantitative analysis, and the performance of the system is illustrated with practical applications to compositional imaging of cardiac muscle.
开发了一种基于同步扫描电子能量损失谱（EELS）和能量色散X射线谱（EDS）的成分成像系统。该系统利用EELS和EDS的联合功能进行纳米级分辨率的定量成分成像。该系统特别适用于但不限于生物学研究，因为它同时提供来自EELS的诸如钙或磷等元素的灵敏图谱以及来自EDS的许多其他元素的图谱。通过在数据采集期间使用图像互相关校正漂移，可防止样品漂移导致的分辨率下降。为实时和/或离线定量分析实现了几个先进功能，并通过对心肌成分成像的实际应用来说明该系统的性能。
