Leon-Velarde Carlos G, Zosherafatein Leila, Odumeru Joseph A
Laboratory Services Division, University of Guelph, 95 Stone Road West, Guelph, ON N1H8J7, Canada.
J Microbiol Methods. 2009 Oct;79(1):13-7. doi: 10.1016/j.mimet.2009.07.003. Epub 2009 Jul 17.
An automated immunomagnetic separation (IMS) and enzyme immunoassay (EIA) was applied to the detection of Salmonella enterica subspecies enterica serotypes from poultry environmental samples. The analytical sensitivity and specificity of the IMS-EIA for 46 S. enterica serotypes and 33 non-salmonellae isolates belonging to 21 different genera were 91.3% and 90.9%, respectively. From post enrichment S. enterica cultures, the limit of detection of the assay was estimated at 10(4)-10(6) CFU/mL. Application of IMS-EIA on 850 naturally contaminated poultry environmental samples achieved 98.4% sensitivity and 96.8% specificity, as compared with a standard culture reference method performed concurrently on the same set of samples. The IMS-EIA described, allows for the identification of suspect positive samples within 48 h of testing versus 4-6 days required by standard culture methods while significantly reducing the materials and labour required for the detection of S. enterica serotypes in poultry environmental samples.
采用自动免疫磁珠分离（IMS）和酶免疫分析（EIA）技术，对家禽环境样本中的肠炎沙门氏菌亚种肠炎血清型进行检测。该IMS-EIA对46种肠炎沙门氏菌血清型及属于21个不同属的33株非沙门氏菌分离株的分析灵敏度和特异性分别为91.3%和90.9%。从增菌后的肠炎沙门氏菌培养物中，该检测方法的检测限估计为10⁴ - 10⁶ CFU/mL。与同时对同一组样本进行的标准培养参考方法相比，将IMS-EIA应用于850份自然污染的家禽环境样本，灵敏度达到98.4%，特异性达到96.8%。所描述的IMS-EIA能够在检测后48小时内鉴定出可疑阳性样本，而标准培养方法则需要4 - 6天，同时显著减少了在家禽环境样本中检测肠炎沙门氏菌血清型所需的材料和劳动力。
