Grzeszczuk Anna, Puzanowska Beata, Miegoć Henryka, Prokopowicz Danuta
Anna Grzeszczuk, MD, Department of Infectious Diseases, Medical University of Bialystok, ul. Zurawia 14, 15-540 Bialystok, Poland.
Ann Agric Environ Med. 2004;11(1):155-7.
The seroprevalence of human granulocytic anaplasmosis (former human granulocytic ehrlichiosis, HGE) has been documented in several studies, but little data exists on incidence rates in healthy individuals. In a prospective study, we tested 125 healthy adults (mean age 43 years)--workers of the Bialowieza Primeval Forest National Park, north-eastern Poland--for Anaplasma phagocytophilum IgG antibodies using an indirect immunofluorescence antibody assay, and for Borrelia burgdorferi IgG with ELISA in a 12-month interval. The data concerning clinical symptoms consistent with human granulocytic anaplasmosis were collected using a standardized questionnaire. Of these 125 subjects, 9 were anti-A. phagocytophilum positive at the study entry. Four participants (3.2 %) seroconverted from IgG negative to positive during the observation period. Three subjects (2.4 %) converted from initially anti-A. phagocytophilum positive to negative. Specific IgG antibodies against Borrelia burgdorferi were detected in 27 (21.6 %) individuals. Concurrence of Borrelia burgdorferi and Anaplasma phagocytophilum was observed in 3.2 %, whereas 4 % were Anaplasma phagocytophilum IgG positive and Borrelia burgdorferi IgG negative (not significant). Clinical symptoms associated with human granulocytic anaplasmosis were not present in seroconverting individuals. The obtained results confirm the occurrence of Anaplasma phagocytophilum infection in north-eastern Poland with asymptomatic clinical course.
多项研究记录了人类粒细胞无形体病(原人类粒细胞埃立克体病,HGE)的血清阳性率,但关于健康个体发病率的数据却很少。在一项前瞻性研究中,我们对125名健康成年人(平均年龄43岁)——波兰东北部比亚沃维耶扎原始森林国家公园的工作人员——每隔12个月使用间接免疫荧光抗体检测法检测嗜吞噬细胞无形体IgG抗体,并使用酶联免疫吸附测定法检测伯氏疏螺旋体IgG。使用标准化问卷收集与人类粒细胞无形体病一致的临床症状数据。在这125名受试者中,9人在研究开始时抗嗜吞噬细胞无形体呈阳性。4名参与者(3.2%)在观察期内从IgG阴性血清转化为阳性。3名受试者(2.4%)从最初的抗嗜吞噬细胞无形体阳性转化为阴性。27名(21.6%)个体检测到抗伯氏疏螺旋体的特异性IgG抗体。观察到3.2%的人同时感染伯氏疏螺旋体和嗜吞噬细胞无形体,而4%的人嗜吞噬细胞无形体IgG呈阳性且伯氏疏螺旋体IgG呈阴性(无显著差异)。血清转化的个体中未出现与人类粒细胞无形体病相关的临床症状。获得的结果证实了波兰东北部存在嗜吞噬细胞无形体感染,且临床病程无症状。