National Institute of Public Health, National Reference Laboratory for Lyme Borreliosis, Prague, Czech Republic.
Vector Borne Zoonotic Dis. 2009 Dec;9(6):655-61. doi: 10.1089/vbz.2008.0127.
The aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with Anaplasma phagocytophilum or Borrelia burgdorferi sensu lato (s. l.) in the Czech Republic. This data can provide information relevant to human infection. In total, blood samples from 296 dogs and 118 engorged ticks were examined. Samples were tested for A. phagocytophilum using polymerase chain reaction (PCR) amplification, nested PCR, and direct sequencing of the 16S rDNA, and for B. burgdorferi s. l. using PCR amplification of the 16S rDNA and restriction fragment length polymorphism analysis of the 5S-23S rDNA intergenic spacer. In addition, blood samples were screened for antibodies to these bacteria. Ten (3.4%) dogs were PCR-positive for A. phagocytophilum. Morulae of A. phagocytophilum in granulocytes were found in two of these dogs. Nine of the PCR-positive dogs had clinical signs related to anaplasmosis. Statistically significant differences in the PCR detection rates were found between breeds and between symptomatic and asymptomatic dogs. Infection with Borrelia garinii was detected by PCR in a dog with meningoencephalitis. DNA of A. phagocytophilum and B. burgdorferi s. l. (B. garinii or Borrelia afzelii) was detected in 8.5% and 6.8% of ticks, respectively. Immunoglobulin (Ig) G seropositivity to A. phagocytophilum was 26%. Significant differences were found with respect to breed and gender. IgM and IgG antibodies to B. burgdorferi s. l. were detected in 2.4% and 10.3% of dogs, respectively. Our findings suggest that the exposure to B. burgdorferi s. l. exists in dogs in the Czech Republic, and exposure to A. phagocytophilum is common.
本研究旨在介绍捷克自然感染嗜吞噬细胞无形体或伯氏疏螺旋体（s. l.）的犬的分子、血清学和临床发现。这些数据可为人类感染提供相关信息。共检查了 296 只犬和 118 只饱血蜱的血液样本。使用聚合酶链反应（PCR）扩增、巢式 PCR 和 16S rDNA 直接测序检测 A. phagocytophilum，使用 PCR 扩增 16S rDNA 和 5S-23S rDNA 基因间隔区的限制性片段长度多态性分析检测 B. burgdorferi s. l.。此外，还对这些细菌的抗体进行了筛查。10 只（3.4%）犬的 A. phagocytophilum PCR 检测结果为阳性。在其中两只犬的粒细胞中发现了嗜吞噬细胞无形体的菌栓。9 只 PCR 阳性犬有与无形体病相关的临床症状。在不同品种和有症状与无症状犬之间，PCR 检测率存在统计学显著差异。PCR 检测到一只患有脑膜脑炎的犬感染了伯氏疏螺旋体格氏亚种。在 8.5%的蜱和 6.8%的蜱中分别检测到 A. phagocytophilum 和 B. burgdorferi s. l.（B. garinii 或伯氏疏螺旋体 afzelii）的 DNA。对 A. phagocytophilum 的 IgG 血清阳性率为 26%。在品种和性别方面存在显著差异。B. burgdorferi s. l. 的 IgM 和 IgG 抗体在 2.4%和 10.3%的犬中被检测到。我们的发现表明，捷克犬中存在伯氏疏螺旋体 s. l. 的暴露，而嗜吞噬细胞无形体的暴露则很常见。
