Miller Kathleen A, Elkind-Hirsch Karen, Levy Brynn, Graubert Michael D, Ross Sudeep J, Scott Richard T
Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07962, USA.
Fertil Steril. 2004 Jul;82(1):211-4. doi: 10.1016/j.fertnstert.2003.12.031.
To describe the successful use of preimplantation genetic diagnosis to assess the prevalence of meiotic errors after oocyte cryopreservation in an oocyte donation cycle.
Case report.
Private IVF center.
PATIENT(S): A 42.6-year-old patient with ovarian failure.
INTERVENTION(S): A donor oocyte IVF cycle with cryopreservation of oocytes followed by thaw, fertilization of oocytes, preimplantation genetic diagnosis for selective aneuploidy, and ET.
MAIN OUTCOME MEASURE(S): Preimplantation genetic analysis of chromosomes 13,16,18, 21,22, X, and Y with fluorescence in-situ hybridization.
RESULT(S): The recipient's initial serum beta-hCG level was 196 mIU/mL 15 days after oocyte retrieval. An initial ultrasound at the sixth week of gestation revealed two gestational sacs. A second ultrasound 1 week later showed a monochorionic twin in sac A and a singleton pregnancy in sac B. Fetal cardiac activity was visualized for all gestations.
CONCLUSION(S): This case illustrates the feasibility of cryopreservation of donor oocytes combined with preimplantation genetic diagnosis for clinical use in those settings where there may be an increased risk of spindle-related abnormalities.
描述在一个供卵周期中，如何成功运用植入前基因诊断来评估卵母细胞冷冻保存后减数分裂错误的发生率。
病例报告。
私立体外受精中心。
一名42.6岁的卵巢功能衰竭患者。
进行供卵体外受精周期，对卵母细胞进行冷冻保存，随后解冻、卵母细胞受精、针对选择性非整倍体进行植入前基因诊断以及胚胎移植。
采用荧光原位杂交技术对13、16、18、21、22号染色体以及X和Y染色体进行植入前基因分析。
取卵后15天，受者血清β - hCG初始水平为196 mIU/mL。妊娠第6周的初次超声检查显示有两个妊娠囊。1周后的第二次超声检查显示，A囊中为单绒毛膜双胎，B囊中为单胎妊娠。所有妊娠均可见胎心搏动。
该病例表明，在可能存在纺锤体相关异常风险增加的情况下，供卵母细胞冷冻保存结合植入前基因诊断用于临床是可行的。
