Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin, 719000, China.
College of Life Sciences, Yulin University, Yulin, 719000, China.
Sci Rep. 2023 Aug 12;13(1):13134. doi: 10.1038/s41598-023-39953-2.
Selenium (Se) is an important microelement for animal health. However, the knowledge about the effects of Se supplementation on rumen eukaryotic community remains less explored. In this study, the ruminal eukaryotic diversity in three months old Shaanbei white cashmere wether goats, with body weight (26.18 ± 2.71) kg, fed a basal diet [0.016 mg/kg Se dry matter (DM), control group (CG)] were compared to those animals given basal diet supplemented with different levels of organic Se in the form of Selenohomolanthionine (SeHLan), namely low Se group (LSE, 0.3 mg/kg DM), medium Se group (MSE, 0.6 mg/kg Se DM) and high Se group (HSE, 1.2 mg/kg DM) using 18S rRNA amplicon sequencing. Illumina sequencing generated 2,623,541 reads corresponding to 3123 operational taxonomic units (OTUs). Taxonomic analysis revealed that Eukaryota (77.95%) and Fungi (14.10%) were the dominant eukaryotic kingdom in all samples. The predominant rumen eukaryotic phylum was found to be Ciliophora (92.14%), while fungal phyla were dominated by Ascomycota (40.77%), Basidiomycota (23.77%), Mucoromycota (18.32%) and unidentified_Fungi (13.89%). The dominant eukaryotic genera were found to be Entodinium (55.44%), Ophryoscolex (10.51%) and Polyplastron (10.19%), while the fungal genera were dominanted by Mucor (15.39%), Pichia (9.88%), Aspergillu (8.24%), Malassezia (7.73%) and unidentified_Neocallimastigaceae (7.72%). The relative abundance of eukaryotic genera Ophryoscolex, Enoploplastron and fungal genus Mucor were found to differ significantly among the four treatment groups (P < 0.05). Moreover, Spearman correlation analysis revealed that the ciliate protozoa and fungi were negatively correlated with each other. The results of this study provided newer information about the effects of Se on rumen eukaryotic diversity patterns using 18s rRNA high-throughput sequencing technology.
硒（Se）是动物健康的重要微量元素。然而，关于硒补充对瘤胃真核生物群落的影响的知识仍有待进一步探索。在这项研究中，我们比较了 3 月龄陕北白绒山羊公羊（体重为 26.18±2.71kg）在基础日粮（硒含量为 0.016mg/kg 干物质，对照组（CG））和基础日粮中添加不同水平的有机硒（以硒同型半胱氨酸的形式）条件下的瘤胃真核生物多样性，低硒组（LSE，0.3mg/kg DM）、中硒组（MSE，0.6mg/kg Se DM）和高硒组（HSE，1.2mg/kg DM）。使用 18S rRNA 扩增子测序。Illumina 测序生成了 2623541 条对应于 3123 个操作分类单位（OTUs）的reads。分类分析表明，Eukaryota（77.95%）和 Fungi（14.10%）是所有样品中占优势的真核生物界。在所有样本中，最主要的瘤胃真核生物门是纤毛门（92.14%），而真菌门主要由子囊菌门（40.77%）、担子菌门（23.77%）、毛霉菌门（18.32%）和未鉴定的真菌门（13.89%）组成。占优势的真核生物属是 Entodinium（55.44%）、Ophryoscolex（10.51%）和 Polyplastron（10.19%），而真菌属主要是 Mucor（15.39%）、Pichia（9.88%）、Aspergillus（8.24%）、Malassezia（7.73%）和未鉴定的 Neocallimastigaceae（7.72%）。四个处理组之间真核生物属 Ophryoscolex、Enoploplastron 和真菌属 Mucor 的相对丰度存在显著差异（P<0.05）。此外，Spearman 相关性分析表明纤毛虫原生动物和真菌之间存在负相关关系。本研究结果利用 18S rRNA 高通量测序技术提供了关于硒对瘤胃真核生物多样性模式影响的新信息。
