基于LightCycler的脓肿分枝杆菌和龟分枝杆菌鉴别
LightCycler-based differentiation of Mycobacterium abscessus and Mycobacterium chelonae.
作者信息
Sedlacek L, Rifai M, Feldmann K, Bange F C
机构信息
Department of Medical Microbiology and Hospital Epidemiology, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
出版信息
J Clin Microbiol. 2004 Jul;42(7):3284-7. doi: 10.1128/JCM.42.7.3284-3287.2004.
Abstract
In this study we introduce a rapid procedure to identify Mycobacterium abscessus (types I and II) and M. chelonae using LightCycler-based analysis of the hsp65 gene. Results from 36 clinical strains were compared with hsp65 gene restriction analysis and biochemical profiles of bacilli. As all three methods yielded identical results for each isolate, this procedure offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnosis of mycobacterial diseases.
摘要
在本研究中，我们介绍了一种快速方法，利用基于LightCycler的hsp65基因分析来鉴定脓肿分枝杆菌（I型和II型）和龟分枝杆菌。将36株临床菌株的结果与hsp65基因限制性分析及杆菌的生化谱进行了比较。由于这三种方法对每一株分离菌都产生了相同的结果，该方法为先前建立的基于核酸扩增的技术用于诊断分枝杆菌病提供了一个极佳的替代方法。
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